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Functional characterization of CNOT3 variants identified in familial adenomatous polyposis adenomas.
Delacruz, Richard Glenn C; Sandoval, Imelda T; Chang, Kyle; Miller, Braden N; Reyes-Uribe, Laura; Borras, Ester; Lynch, Patrick M; Taggart, Melissa W; Hawk, Ernest T; Vilar, Eduardo; Jones, David A.
Afiliación
  • Delacruz RGC; Functional and Chemical Genomics, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.
  • Sandoval IT; Functional and Chemical Genomics, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.
  • Chang K; Department of Clinical Cancer Prevention, The University of Texas UTHealth MD Anderson Cancer Center, Houston, TX, USA.
  • Miller BN; Graduate School of Biomedical Sciences, The University of Texas UTHealth MD Anderson Cancer Center, Houston, TX, USA.
  • Reyes-Uribe L; Functional and Chemical Genomics, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.
  • Borras E; College of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
  • Lynch PM; Department of Clinical Cancer Prevention, The University of Texas UTHealth MD Anderson Cancer Center, Houston, TX, USA.
  • Taggart MW; Department of Clinical Cancer Prevention, The University of Texas UTHealth MD Anderson Cancer Center, Houston, TX, USA.
  • Hawk ET; Department of Gastroenterology, Hepatology and Nutrition, The University of Texas UTHealth MD Anderson Cancer Center, Houston, TX, USA.
  • Vilar E; Clinical Genetics Program, The University of Texas UTHealth MD Anderson Cancer Center, Houston, TX, USA.
  • Jones DA; Department of Pathology, The University of Texas UTHealth MD Anderson Cancer Center, Houston, TX, USA.
Oncotarget ; 10(39): 3939-3951, 2019 Jun 11.
Article en En | MEDLINE | ID: mdl-31231471
Germline mutations in the tumor suppressor Adenomatous Polyposis Coli (APC) define Familial Adenomatous Polyposis (FAP), the genetic predisposition to developing adenomatous polyps. Recent sequencing of FAP adenomas have challenged established dogma that APC mutations alone represent the adenoma mutational landscape because recurrent somatic mutations in non-WNT pathway genes were also discovered. In particular, one of these novel genes, CNOT3, presented E20K and E70K mutations that are predicted to be deleterious in silico. We utilized zebrafish embryos to determine if these mutations affect CNOT3 function and perform novel biology in an APC-dependent pathway in vivo. Human CNOT3 (hCNOT3) and E20K mRNA injection rescued zebrafish cnot3a knockdown lordosis phenotype while E70K did not. In the FAP apcmcr zebrafish model, we show that ctbp1, but not retinoic acid, regulates cnot3a expression. Injection of hCNOT3 and E20K, but not E70K, to homozygous apcmcr zebrafish initiated gut differentiation while cnot3a knockdown in wildtype embryos led to decreased intestinal development and differentiation. Finally, targeted sequencing of 37 additional FAP adenomas revealed CNOT3 mutations in 20% of these samples. Overall, our work supports a mechanism where CTBP1 regulates CNOT3 and that overall CNOT3 perturbation could work in concert with germline APC mutations in advancing adenomas to a more transformed state prior to progression to adenocarcinoma.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Oncotarget Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Oncotarget Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos