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A New Strategy for the Detection of Chicken Infectious Anemia Virus Contamination in Attenuated Live Vaccine by Droplet Digital PCR.
Li, Qiuchen; Zhang, Yubiao; Meng, Fanfeng; Jiang, Hui; Xu, Guanlong; Ding, Jiabo; Zhang, Yawen; Dong, Guiwei; Tian, Sibao; Chang, Shuang; Zhao, Peng.
Afiliación
  • Li Q; College of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong, China.
  • Zhang Y; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, Shandong, China.
  • Meng F; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, Shandong, China.
  • Jiang H; Yuyao Animal Husbandry and Veterinary Bureau, Ningbo, Zhejiang, China.
  • Xu G; Beijing Dafaun Poultry Breeding Company Ltd., Beijing, China.
  • Ding J; National Reference Laboratory for Animal Brucellosis, China Institute of Veterinary Drug Control, Beijing 102629, China.
  • Zhang Y; National Reference Laboratory for Animal Brucellosis, China Institute of Veterinary Drug Control, Beijing 102629, China.
  • Dong G; National Reference Laboratory for Animal Brucellosis, China Institute of Veterinary Drug Control, Beijing 102629, China.
  • Tian S; College of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong, China.
  • Chang S; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, Shandong, China.
  • Zhao P; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, Shandong, China.
Biomed Res Int ; 2019: 2750472, 2019.
Article en En | MEDLINE | ID: mdl-31223613
Chicken infectious anemia virus (CIAV) causes the atrophy of bone marrow hematopoietic and lymphoid tissues in chicks, leading to huge economic losses all over the world. The using of attenuated vaccine contaminated with CIAV increased the mortality and the pathogenicity of other diseases in many farms. However, it is difficult to detect the CIAV contamination by general detection technology due to the extremely low dose of CIAV in vaccines. In this study, we established a new method called droplet digital Polymerase Chain Reaction (ddPCR) to detect CIAV contamination of vaccines more sensitively and accurately. The lowest detection limitation of this method is 2.4 copies of CIAV plasmid or CIAV contamination at 0.1 EID50/1000 feathers in vaccines without any positive signals of other viruses. Besides, the sensitivity of ddPCR is 100 times greater than that of conventional PCR and 10 times greater than that of real-time PCR. The ddPCR technique is more sensitive and more intuitive. Therefore, it could be valuable for the detection of CIAV contamination in vaccines.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vacunas Virales / Pollos / Reacción en Cadena de la Polimerasa / Contaminación de Medicamentos / Virus de la Anemia del Pollo Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Biomed Res Int Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vacunas Virales / Pollos / Reacción en Cadena de la Polimerasa / Contaminación de Medicamentos / Virus de la Anemia del Pollo Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Biomed Res Int Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos