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Optical crosstalk in SPAD arrays for high-throughput single-molecule fluorescence spectroscopy.
Ingargiola, Antonino; Segal, Maya; Gulinatti, Angelo; Rech, Ivan; Labanca, Ivan; Maccagnani, Piera; Ghioni, Massimo; Weiss, Shimon; Michalet, Xavier.
Afiliación
  • Ingargiola A; Department of Chemistry and Biochemistry, University of California Los Angeles, USA.
  • Segal M; Department of Chemistry and Biochemistry, University of California Los Angeles, USA.
  • Gulinatti A; Dip. di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, Milan, Italy.
  • Rech I; Dip. di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, Milan, Italy.
  • Labanca I; Dip. di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, Milan, Italy.
  • Maccagnani P; Istituto per la Microelettronica e Microsistemi, IMM-CNR, Bologna, Italy.
  • Ghioni M; Dip. di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, Milan, Italy.
  • Weiss S; Department of Chemistry and Biochemistry, University of California Los Angeles, USA.
  • Michalet X; Department of Chemistry and Biochemistry, University of California Los Angeles, USA.
Nucl Instrum Methods Phys Res A ; 9(12): 255-258, 2018 Dec 21.
Article en En | MEDLINE | ID: mdl-31223178
Single-molecule fluorescence spectroscopy (SMFS), based on the detection of individual molecules freely diffusing through the excitation spot of a confocal microscope, has allowed unprecedented insights into biological processes at the molecular level, but suffers from limited throughput. We have recently introduced a multispot version of SMFS, which allows achieving high-throughput SMFS by virtue of parallelization, and relies on custom silicon single-photon avalanche diode (SPAD) detector arrays. Here, we examine the premise of this parallelization approach, which is that data acquired from different spots is uncorrelated. In particular, we measure the optical crosstalk characteristics of the two 48-pixel SPAD arrays used in our recent SMFS studies, and demonstrate that it is negligible (crosstalk probability ≤ 1.1 10-3) and undetectable in cross-correlation analysis of actual single-molecule fluorescence data.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Nucl Instrum Methods Phys Res A Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Nucl Instrum Methods Phys Res A Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos