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Discovery of Potential Anti-infective Therapy Targeting Glutamine Synthetase in Staphylococcus xylosus.
Cui, Wen-Qiang; Qu, Qian-Wei; Wang, Jin-Peng; Bai, Jing-Wen; Bello-Onaghise, God'spower; Li, Yu-Ang; Zhou, Yong-Hui; Chen, Xing-Ru; Liu, Xin; Zheng, Si-Di; Xing, Xiao-Xu; Eliphaz, Nsabimana; Li, Yan-Hua.
Afiliación
  • Cui WQ; College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
  • Qu QW; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, China.
  • Wang JP; College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
  • Bai JW; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, China.
  • Bello-Onaghise G; College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
  • Li YA; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, China.
  • Zhou YH; College of Science, Northeast Agricultural University, Harbin, China.
  • Chen XR; College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
  • Liu X; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, China.
  • Zheng SD; College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
  • Xing XX; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, China.
  • Eliphaz N; College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
  • Li YH; Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, China.
Front Chem ; 7: 381, 2019.
Article en En | MEDLINE | ID: mdl-31214565
Glutamine synthetase (GS), which catalyzes the production of glutamine, plays essential roles in most biological growth and biofilm formation, suggesting that GS may be used as a promising target for antibacterial therapy. We asked whether a GS inhibitor could be found as an anti-infective agent of Staphylococcus xylosus (S. xylosus). Here, computational prediction followed by experimental testing was used to characterize GS. Sorafenib was finally determined through computational prediction. In vitro experiments showed that sorafenib has an inhibitory effect on the growth of S. xylosus by competitively occupying the active site of GS, and the minimum inhibitory concentration was 4 mg/L. In vivo experiments also proved that treatment with sorafenib significantly reduced the levels of TNF-α and IL-6 in breast tissue from mice mastitis, which was further confirmed by histopathology examination. These findings indicated that sorafenib could be utilized as an anti-infective agent for the treatment of infections caused by S. xylosus.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Front Chem Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Front Chem Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza