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Host Immune Response to ZIKV in an Immunocompetent Embryonic Mouse Model of Intravaginal Infection.
Khaiboullina, Svetlana F; Lopes, Priscila; de Carvalho, Toniana G; Real, Ana Luiza C V; Souza, Danielle G; Costa, Vivian V; Teixeira, Mauro M; Bloise, Enrrico; Verma, Subhash C; Ribeiro, Fabiola M.
Afiliación
  • Khaiboullina SF; Department of Microbiology and Immunology, University of Nevada, Reno, NV 89557, USA. sv.khaiboullina@gmail.com.
  • Lopes P; Department of Biochemistry and Immunology, Institute of Biological Sciences (ICB), Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil. pripsgraz@gmail.com.
  • de Carvalho TG; Department of Biochemistry and Immunology, Institute of Biological Sciences (ICB), Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil. toniana_gc@hotmail.com.
  • Real ALCV; Department of Biochemistry and Immunology, Institute of Biological Sciences (ICB), Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil. analuza321@gmail.com.
  • Souza DG; Department of Microbiology, Institute of Biological Sciences (ICB), Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil. souzadg@gmail.com.
  • Costa VV; Department of Morphology, Institute of Biological Sciences (ICB), Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil. vivianvcosta@gmail.com.
  • Teixeira MM; Department of Biochemistry and Immunology, Institute of Biological Sciences (ICB), Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil. mmtex.ufmg@gmail.com.
  • Bloise E; Department of Morphology, Institute of Biological Sciences (ICB), Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil. enrico_bloise@hotmail.com.
  • Verma SC; Department of Microbiology and Immunology, University of Nevada, Reno, NV 89557, USA. fmribeiro@icb.ufmg.br.
  • Ribeiro FM; Department of Biochemistry and Immunology, Institute of Biological Sciences (ICB), Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil. scverma@medicine.nevada.edu.
Viruses ; 11(6)2019 06 17.
Article en En | MEDLINE | ID: mdl-31212905
Zika virus (ZIKV) only induces mild symptoms in adults; however, it can cause congenital Zika syndrome (CZS), including microcephaly. Most of the knowledge on ZIKV pathogenesis was gained using immunocompromised mouse models, which do not fully recapitulate human pathology. Moreover, the study of the host immune response to ZIKV becomes challenging in these animals. Thus, the main goal of this study was to develop an immunocompetent mouse model to study the ZIKV spread and teratogeny. FVB/NJ immune competent dams were infected intravaginally with ZIKV during the early stage of pregnancy. We found that the placentae of most fetuses were positive for ZIKV, while the virus was detected in the brain of only about 42% of the embryos. To investigate the host immune response, we measured the expression of several inflammatory factors. Embryos from ZIKV-infected dams had an increased level of inflammatory factors, as compared to Mock. Next, we compared the gene expression levels in embryos from ZIKV-infected dams that were either negative or positive for ZIKV in the brain. The mRNA levels of viral response genes and cytokines were increased in both ZIKV-positive and negative brains. Interestingly, the levels of chemokines associated with microcephaly in humans, including CCL2 and CXCL10, specifically increased in embryos harboring ZIKV in the embryo brains.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Complicaciones Infecciosas del Embarazo / Modelos Animales de Enfermedad / Interacciones Huésped-Patógeno / Virus Zika / Infección por el Virus Zika Tipo de estudio: Prognostic_studies Límite: Animals / Pregnancy Idioma: En Revista: Viruses Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Complicaciones Infecciosas del Embarazo / Modelos Animales de Enfermedad / Interacciones Huésped-Patógeno / Virus Zika / Infección por el Virus Zika Tipo de estudio: Prognostic_studies Límite: Animals / Pregnancy Idioma: En Revista: Viruses Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza