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Development of American-Lineage Influenza H5N2 Reassortant Vaccine Viruses for Pandemic Preparedness.
Chen, Po-Ling; Hu, Alan Yung-Chih; Lin, Chun-Yang; Weng, Tsai-Chuan; Lai, Chia-Chun; Tseng, Yu-Fen; Cheng, Ming-Chu; Chia, Min-Yuan; Lin, Wen-Chin; Yeh, Chia-Tsui; Su, Ih-Jen; Lee, Min-Shi.
Afiliación
  • Chen PL; National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. letitia@nhri.org.tw.
  • Hu AY; Institute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu 30013, Taiwan. letitia@nhri.org.tw.
  • Lin CY; National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. alanhu@nhri.org.tw.
  • Weng TC; National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. grayingaries@outlook.com.
  • Lai CC; National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. wtc@nhri.org.tw.
  • Tseng YF; National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. laicc2@nhri.org.tw.
  • Cheng MC; College of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan. laicc2@nhri.org.tw.
  • Chia MY; National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. yufents@gmail.com.
  • Lin WC; Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan. mccheng@mail.npust.edu.tw.
  • Yeh CT; Animal Health Research Institutes, Danshui, New Taipei City 25158, Taiwan. mccheng@mail.npust.edu.tw.
  • Su IJ; National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, Taiwan. chiaminyuan@dragon.nchu.edu.tw.
  • Lee MS; Department of Veterinary Medicine, National Chung Hsing University, Taichung 40227, Taiwan. chiaminyuan@dragon.nchu.edu.tw.
Viruses ; 11(6)2019 06 11.
Article en En | MEDLINE | ID: mdl-31212631
Novel low-pathogenic avian influenza (LPAI) H5N2 viruses hit poultry farms in Taiwan in 2003, and evolved into highly pathogenic avian influenza (HPAI) viruses in 2010. These viruses are reassortant viruses containing HA and NA genes from American-lineage H5N2 and six internal genes from local H6N1 viruses. According to a serological survey, the Taiwan H5N2 viruses can cause asymptomatic infections in poultry workers. Therefore, a development of influenza H5N2 vaccines is desirable for pandemic preparation. In this study, we employed reverse genetics to generate a vaccine virus having HA and NA genes from A/Chicken/CY/A2628/2012 (E7, LPAI) and six internal genes from a Vero cell-adapted high-growth H5N1 vaccine virus (Vero-15). The reassortant H5N2 vaccine virus, E7-V15, presented high-growth efficiency in Vero cells (512 HAU, 107.6 TCID50/mL), and passed all tests for qualification of candidate vaccine viruses. In ferret immunization, two doses of inactivated whole virus antigens (3 µg of HA protein) adjuvanted with alum could induce robust antibody response (HI titre 113.14). In conclusion, we have established reverse genetics to generate a qualified reassortant H5N2 vaccine virus for further development.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vacunas contra la Influenza / Virus Reordenados / Gripe Humana / Subtipo H5N2 del Virus de la Influenza A Límite: Animals / Humans País/Región como asunto: Asia Idioma: En Revista: Viruses Año: 2019 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vacunas contra la Influenza / Virus Reordenados / Gripe Humana / Subtipo H5N2 del Virus de la Influenza A Límite: Animals / Humans País/Región como asunto: Asia Idioma: En Revista: Viruses Año: 2019 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Suiza