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Accelerating Drug Discovery by Early Protein Drug Target Prediction Based on a Multi-Fingerprint Similarity Search.
Montaruli, Michele; Alberga, Domenico; Ciriaco, Fulvio; Trisciuzzi, Daniela; Tondo, Anna Rita; Mangiatordi, Giuseppe Felice; Nicolotti, Orazio.
Afiliación
  • Montaruli M; Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari "Aldo Moro", via E. Orabona, 4, I-70125 Bari, Italy. michele.montaruli@gmail.com.
  • Alberga D; Cineca, Via Magnanelli 6/3, 40033 Casalecchio di Reno, Bologna, Italy. d.alberga@cineca.it.
  • Ciriaco F; Dipartimento di Chimica, Università degli Studi di Bari "Aldo Moro", via E. Orabona, 4, I-70125 Bari, Italy. fulvio.ciriaco@uniba.it.
  • Trisciuzzi D; Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari "Aldo Moro", via E. Orabona, 4, I-70125 Bari, Italy. daniela.trisciuzzi@uniba.it.
  • Tondo AR; Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Via la Masa 19, 20156 Milano, Italy. annarita.tondo@gmail.com.
  • Mangiatordi GF; Istituto di Cristallografia, Consiglio Nazionale delle Ricerche, Via G. Amendola 122/O, 70126 Bari, Italy. giuseppe.mangiatordi@ic.cnr.it.
  • Nicolotti O; Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari "Aldo Moro", via E. Orabona, 4, I-70125 Bari, Italy. orazio.nicolotti@uniba.it.
Molecules ; 24(12)2019 Jun 14.
Article en En | MEDLINE | ID: mdl-31207991
In this continuing work, we have updated our recently proposed Multi-fingerprint Similarity Search algorithm (MuSSel) by enabling the generation of dominant ionized species at a physiological pH and the exploration of a larger data domain, which included more than half a million high-quality small molecules extracted from the latest release of ChEMBL (version 24.1, at the time of writing). Provided with a high biological assay confidence score, these selected compounds explored up to 2822 protein drug targets. To improve the data accuracy, samples marked as prodrugs or with equivocal biological annotations were not considered. Notably, MuSSel performances were overall improved by using an object-relational database management system based on PostgreSQL. In order to challenge the real effectiveness of MuSSel in predicting relevant therapeutic drug targets, we analyzed a pool of 36 external bioactive compounds published in the Journal of Medicinal Chemistry from October to December 2018. This study demonstrates that the use of highly curated chemical and biological experimental data on one side, and a powerful multi-fingerprint search algorithm on the other, can be of the utmost importance in addressing the fate of newly conceived small molecules, by strongly reducing the attrition of early phases of drug discovery programs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas / Modelos Moleculares / Descubrimiento de Drogas / Modelos Químicos Tipo de estudio: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas / Modelos Moleculares / Descubrimiento de Drogas / Modelos Químicos Tipo de estudio: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza