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Comparative analysis of in vitro proliferative, migratory and pro-angiogenic potentials of bovine fetal mesenchymal stem cells derived from bone marrow and adipose tissue.
Jervis, M; Huaman, O; Cahuascanco, B; Bahamonde, J; Cortez, J; Arias, J I; Torres, C G; Peralta, O A.
Afiliación
  • Jervis M; Department of Animal Production Science, Faculty of Veterinary Sciences, University of Chile, 8820808, Santiago, Chile.
  • Huaman O; Department of Animal Production Science, Faculty of Veterinary Sciences, University of Chile, 8820808, Santiago, Chile.
  • Cahuascanco B; Department of Animal Production Science, Faculty of Veterinary Sciences, University of Chile, 8820808, Santiago, Chile.
  • Bahamonde J; Department of Animal Production Science, Faculty of Veterinary Sciences, University of Chile, 8820808, Santiago, Chile.
  • Cortez J; Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, VA, 24060, USA.
  • Arias JI; Institute of Pharmacology and Morphophysiology, Faculty of Veterinary Sciences, Austral University of Chile, 5110566, Valdivia, Chile.
  • Torres CG; Department of Animal Production Science, Faculty of Veterinary Sciences, University of Chile, 8820808, Santiago, Chile.
  • Peralta OA; Department of Clinical Science, Faculty of Veterinary Sciences, University of Chile, 8820808, Santiago, Chile.
Vet Res Commun ; 43(3): 165-178, 2019 Aug.
Article en En | MEDLINE | ID: mdl-31201618
Mesenchymal stem cells (MSCs) are found in virtually all tissues, where they self-renew and differentiate into multiple cell types. Cumulative data indicate that MSCs secrete paracrine factors that may play key roles in the treatment of various acute and chronic pathological conditions in diverse animal species including cattle. The aim of the present study was to compare the potentials for proliferation, migration and pro-angiogenesis of bovine fetal BM-MSCs and AT-MSCs under in vitro conditions. Growth curves and population doubling time (PDT) were determined for BM-MSCs and AT-MSCs in order to compare in vitro cell proliferation potentials. The ability of BM-MSCs and AT-MSCs to migrate was evaluated by scratch plate and transwell migration assays. The pro-angiogenic potential of conditioned medium from BM-MSCs and AT-MSCs was compared using an endothelial cell (EC) tubule formation assay. BM-MSCs displayed higher proliferation curves and doubled their populations in fewer days compared to AT-MSCs. No significant differences were detected in the number of migrant cells between BM-MSCs and AT-MSCs; however, a higher migration value was detected for BM-MSCs compared to fibroblasts (FBs), and a higher number of migrant cells were attracted by DMEM supplemented with 5% fetal bovine serum (FBS) compared to stromal cell-derived factor-1 (SDF-1). More tubules of ECs were formed after exposure to concentrated conditioned medium from AT-MSCs compared to BM-MSCs, FBs or DMEM controls. Despite common mesodermal origin, BM-MSCs display higher proliferative capacity and lower pro-angiogenic potential compared to AT-MSCs; however, both cell types possess similar migratory ability.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células de la Médula Ósea / Tejido Adiposo / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: Vet Res Commun Año: 2019 Tipo del documento: Article País de afiliación: Chile Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células de la Médula Ósea / Tejido Adiposo / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: Vet Res Commun Año: 2019 Tipo del documento: Article País de afiliación: Chile Pais de publicación: Suiza