Your browser doesn't support javascript.
loading
A multivalent Kaposi sarcoma-associated herpesvirus-like particle vaccine capable of eliciting high titers of neutralizing antibodies in immunized rabbits.
Mulama, David H; Mutsvunguma, Lorraine Z; Totonchy, Jennifer; Ye, Peng; Foley, Joslyn; Escalante, Gabriela M; Rodriguez, Esther; Nabiee, Ramina; Muniraju, Murali; Wussow, Felix; Barasa, Anne K; Ogembo, Javier Gordon.
Afiliación
  • Mulama DH; Department of Immuno-Oncology, Beckman Research Institute of City of Hope, Duarte, CA, United States; Biological Sciences Department, Masinde Muliro University of Science and Technology, Kakamega, Kenya.
  • Mutsvunguma LZ; Department of Immuno-Oncology, Beckman Research Institute of City of Hope, Duarte, CA, United States.
  • Totonchy J; Chapman University, School of Pharmacy, Irvine, CA, United States.
  • Ye P; Department of Immuno-Oncology, Beckman Research Institute of City of Hope, Duarte, CA, United States.
  • Foley J; Department of Immuno-Oncology, Beckman Research Institute of City of Hope, Duarte, CA, United States.
  • Escalante GM; Irell & Manella Graduate School of Biological Sciences of City of Hope, Duarte, CA, United States.
  • Rodriguez E; Department of Immuno-Oncology, Beckman Research Institute of City of Hope, Duarte, CA, United States.
  • Nabiee R; Chapman University, School of Pharmacy, Irvine, CA, United States.
  • Muniraju M; Department of Immuno-Oncology, Beckman Research Institute of City of Hope, Duarte, CA, United States.
  • Wussow F; Department of Immuno-Oncology, Beckman Research Institute of City of Hope, Duarte, CA, United States.
  • Barasa AK; Department of Immuno-Oncology, Beckman Research Institute of City of Hope, Duarte, CA, United States; Department of Human Pathology, University of Nairobi, Nairobi, Kenya.
  • Ogembo JG; Department of Immuno-Oncology, Beckman Research Institute of City of Hope, Duarte, CA, United States. Electronic address: jogembo@coh.org.
Vaccine ; 37(30): 4184-4194, 2019 07 09.
Article en En | MEDLINE | ID: mdl-31201053
Kaposi sarcoma-associated herpesvirus (KSHV) is an emerging pathogen and the causative agent of multiple cancers in immunocompromised patients. To date, there is no licensed prophylactic KSHV vaccine. In this study, we generated a novel subunit vaccine that incorporates four key KSHV envelope glycoproteins required for viral entry in diverse cell types (gpK8.1, gB, and gH/gL) into a single multivalent KSHV-like particle (KSHV-LP). Purified KSHV-LPs were similar in size, shape, and morphology to KSHV virions. Vaccination of rabbits with adjuvanted KSHV-LPs generated strong glycoprotein-specific antibody responses, and purified immunoglobulins from KSHV-LP-immunized rabbits neutralized KSHV infection in epithelial, endothelial, fibroblast, and B cell lines (60-90% at the highest concentration tested). These findings suggest that KSHV-LPs may be an ideal platform for developing a safe and effective prophylactic KSHV vaccine. We envision performing future studies in animal models that are susceptible to KSHV infection, to determine correlates of immune protection in vivo.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Herpesvirus Humano 8 / Anticuerpos Neutralizantes Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Animals Idioma: En Revista: Vaccine Año: 2019 Tipo del documento: Article País de afiliación: Kenia Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Herpesvirus Humano 8 / Anticuerpos Neutralizantes Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Animals Idioma: En Revista: Vaccine Año: 2019 Tipo del documento: Article País de afiliación: Kenia Pais de publicación: Países Bajos