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MoS2 Field-Effect Transistor-Amyloid-ß1-42 Hybrid Device for Signal Amplified Detection of MMP-9.
Park, Heekyeong; Lee, Hyungbeen; Jeong, Seok Hwan; Lee, Eunjin; Lee, Wonseok; Liu, Na; Yoon, Dae Sung; Kim, Sunkook; Lee, Sang Woo.
Afiliación
  • Park H; School of Advanced Materials Science and Engineering , Sungkyunkwan University (SKKU) , Suwon , Kyunggi-do 16419 , Republic of Korea.
  • Lee H; Department of Biomedical Engineering , Yonsei University , Wonju , Gangwon-do 26493 , Republic of Korea.
  • Jeong SH; School of Advanced Materials Science and Engineering , Sungkyunkwan University (SKKU) , Suwon , Kyunggi-do 16419 , Republic of Korea.
  • Lee E; Department of Biomedical Engineering , Yonsei University , Wonju , Gangwon-do 26493 , Republic of Korea.
  • Lee W; Department of Biomedical Engineering , Yonsei University , Wonju , Gangwon-do 26493 , Republic of Korea.
  • Liu N; School of Advanced Materials Science and Engineering , Sungkyunkwan University (SKKU) , Suwon , Kyunggi-do 16419 , Republic of Korea.
  • Yoon DS; School of Biomedical Engineering , Korea University , Seoul , 02841 , Republic of Korea.
  • Kim S; School of Advanced Materials Science and Engineering , Sungkyunkwan University (SKKU) , Suwon , Kyunggi-do 16419 , Republic of Korea.
  • Lee SW; Department of Biomedical Engineering , Yonsei University , Wonju , Gangwon-do 26493 , Republic of Korea.
Anal Chem ; 91(13): 8252-8258, 2019 07 02.
Article en En | MEDLINE | ID: mdl-31192581
The detection of circulating protein (CP) is very important for the diagnosis and therapeutics of cancer. Conventional techniques based on a specific antibody-antigen interaction are still lacking because of a shortage of cost effectiveness, complicated sandwich structure and tagging process, and inconsistent detection of CP due to the inherent instability of antibodies. Herein, we demonstrate a hybrid device consisting of two-dimensional (2D) nanoscale molybdenum disulfide (MoS2) field-effect transistor (FET) with an amyloid-ß1-42 (Aß1-42) functionalized surface, which amplifies electric signals of the FET in order to detect matrix metalloproteinase-9 (MMP-9), which is a certain type of CP that degrades Aß1-42. With the hybrid device, we detected the concentrations of MMP-9 in the range from 1 pM to 10 nM. Moreover, using tapping-mode atomic force microscopy and Kelvin probe force microscopy, we verified that the signal amplification corresponding to the MMP-9 concentrations was caused by the reduced length and the decreased surface potential of degraded Aß1-42 due to MMP-9. The hybrid device studied in this paper can be very useful for monitoring MMP-9 activity, as well as serving as a sensing platform for the electrical signal amplification of 2D MoS2 FET-biosensors.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fragmentos de Péptidos / Transistores Electrónicos / Péptidos beta-Amiloides / Metaloproteinasa 9 de la Matriz / Disulfuros / Molibdeno Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Anal Chem Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fragmentos de Péptidos / Transistores Electrónicos / Péptidos beta-Amiloides / Metaloproteinasa 9 de la Matriz / Disulfuros / Molibdeno Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Anal Chem Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos