Bioconjugation as a smart immobilization approach for &#945;-amylase enzyme using stimuli-responsive Eudragit-L100 polymer: a robust biocatalyst for applications in pharmaceutical industry.

Abdel-Mageed, Heidi Mohamed; Radwan, Rasha Ali; AbuelEzz, Nermeen Zakaria; Nasser, Hebatallah Ahmed; El Shamy, Aliaa Ali; Abdelnaby, Rana M; El Gohary, Nesrine Abdelrehim

Bioconjugation as a smart immobilization approach for α-amylase enzyme using stimuli-responsive Eudragit-L100 polymer: a robust biocatalyst for applications in pharmaceutical industry.

Abdel-Mageed, Heidi Mohamed; Radwan, Rasha Ali; AbuelEzz, Nermeen Zakaria; Nasser, Hebatallah Ahmed; El Shamy, Aliaa Ali; Abdelnaby, Rana M; El Gohary, Nesrine Abdelrehim.

Afiliación

Abdel-Mageed HM; a Molecular Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre , Cairo , Egypt.
Radwan RA; b Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in Egypt (FUE) , Cairo , Egypt.
AbuelEzz NZ; c Biochemistry and Biotechnology Department, Faculty of Pharmacy and Drug Technology, Heliopolis University , Cairo , Egypt.
Nasser HA; d Biochemistry Department, College of Pharmaceutical Sciences & Drug Manufacturing, Misr University for Science and Technology , Cairo , Egypt.
El Shamy AA; e Microbiology and Public Health Department, Faculty of Pharmacy and Drug Technology, Heliopolis University , Egypt.
Abdelnaby RM; e Microbiology and Public Health Department, Faculty of Pharmacy and Drug Technology, Heliopolis University , Egypt.
El Gohary NA; f Pharmaceutical Chemistry Department, Faculty of Pharmacy and Drug Technology, Heliopolis University , Egypt.

Artif Cells Nanomed Biotechnol ; 47(1): 2361-2368, 2019 Dec.

Article en En | MEDLINE | ID: mdl-31190563

Asunto(s)

Acrilatos/química; Biocatálisis; Industria Farmacéutica; Enzimas Inmovilizadas/química; Enzimas Inmovilizadas/metabolismo; Polímeros/química; alfa-Amilasas/química; alfa-Amilasas/metabolismo; Animales; Costos y Análisis de Costo; Industria Farmacéutica/economía; Estabilidad de Enzimas; Concentración de Iones de Hidrógeno; Cinética; Temperatura

Palabras clave

Biocatalyst; Eudragit stimuli-responsive polymer; Immobilized enzymes stability; carbodiimide bio-conjugation; covalent immobilization; starch processing; α-amylase enzyme

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Acrilatos / Industria Farmacéutica / Enzimas Inmovilizadas / Alfa-Amilasas / Biocatálisis Tipo de estudio: Health_economic_evaluation Límite: Animals Idioma: En Revista: Artif Cells Nanomed Biotechnol Año: 2019 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Reino Unido

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