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Effect of antimicrobial nanocomposites on Vibrio cholerae lifestyles: Pellicle biofilm, planktonic and surface-attached biofilm.
Meza-Villezcas, Anaid; Gallego-Hernández, Ana L; Yildiz, Fitnat H; Jaime-Acuña, Oscar E; Raymond-Herrera, Oscar; Huerta-Saquero, Alejandro.
Afiliación
  • Meza-Villezcas A; Centro de Investigación Científica y de Educación Superior de Ensenada, Ensenada, Baja California, México.
  • Gallego-Hernández AL; Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada, Baja California, México.
  • Yildiz FH; Microbiology and Environmental Toxicology Department, University of California Santa Cruz, Santa Cruz, California, United States of America.
  • Jaime-Acuña OE; Microbiology and Environmental Toxicology Department, University of California Santa Cruz, Santa Cruz, California, United States of America.
  • Raymond-Herrera O; Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada, Baja California, México.
  • Huerta-Saquero A; Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada, Baja California, México.
PLoS One ; 14(6): e0217869, 2019.
Article en En | MEDLINE | ID: mdl-31188854
Vibrio cholerae is an important human pathogen causing intestinal disease with a high incidence in developing countries. V. cholerae can switch between planktonic and biofilm lifestyles. Biofilm formation is determinant for transmission, virulence and antibiotic resistance. Due to the enhanced antibiotic resistance observed by bacterial pathogens, antimicrobial nanomaterials have been used to combat infections by stopping bacterial growth and preventing biofilm formation. In this study, the effect of the nanocomposites zeolite-embedded silver (Ag), copper (Cu), or zinc (Zn) nanoparticles (NPs) was evaluated in V. cholerae planktonic cells, and in two biofilm states: pellicle biofilm (PB), formed between air-liquid interphase, and surface-attached biofilm (SB), formed at solid-liquid interfaces. Each nanocomposite type had a distinctive antimicrobial effect altering each V. cholerae lifestyles differently. The ZEO-AgNPs nanocomposite inhibited PB formation at 4 µg/ml, and prevented SB formation and eliminated planktonic cells at 8 µg/ml. In contrast, the nanocomposites ZEO-CuNPs and ZEO-ZnNPs affect V. cholerae viability but did not completely avoid bacterial growth. At transcriptional level, depending on the nanoparticles and biofilm type, nanocomposites modified the relative expression of the vpsL, rbmA and bap1, genes involved in biofilm formation. Furthermore, the relative abundance of the outer membrane proteins OmpT, OmpU, OmpA and OmpW also differs among treatments in PB and SB. This work provides a basis for further study of the nanomaterials effect at structural, genetic and proteomic levels to understand the response mechanisms of V. cholerae against metallic nanoparticles.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plancton / Vibrio cholerae / Regulación Bacteriana de la Expresión Génica / Biopelículas / Nanocompuestos / Nanopartículas del Metal / Antibacterianos Límite: Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plancton / Vibrio cholerae / Regulación Bacteriana de la Expresión Génica / Biopelículas / Nanocompuestos / Nanopartículas del Metal / Antibacterianos Límite: Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos