Your browser doesn't support javascript.
loading
Enhancement of ABA Sensitivity Through Conditional Expression of the ARF10 Gene in Brassica juncea Reveals Fertile Plants with Tolerance Against Alternaria brassicicola.
Mukherjee, Amrita; Mazumder, Mrinmoy; Jana, Jagannath; Srivastava, Archana Kumari; Mondal, Banani; De, Aishee; Ghosh, Swagata; Saha, Upala; Bose, Rahul; Chatterjee, Subhrangsu; Dey, Nrisingha; Basu, Debabrata.
Afiliación
  • Mukherjee A; Division of Plant Biology, Bose Institute, Centenary Campus P-1/12 C.I.T., Scheme-VIIM Kolkata, 700054, West Bengal, India.
  • Mazumder M; Division of Plant Biology, Bose Institute, Centenary Campus P-1/12 C.I.T., Scheme-VIIM Kolkata, 700054, West Bengal, India.
  • Jana J; Division of Plant Biology, Bose Institute, Centenary Campus P-1/12 C.I.T., Scheme-VIIM Kolkata, 700054, West Bengal, India.
  • Srivastava AK; Institut Curie, CNRS UMR 3348, Orsay, France.
  • Mondal B; Plant and Microbial biotechnology, Institute of Life Sciences (ILS), NALCO Square, Bhubaneswar, 751023, Odisha, India.
  • De A; Division of Plant Biology, Bose Institute, Centenary Campus P-1/12 C.I.T., Scheme-VIIM Kolkata, 700054, West Bengal, India.
  • Ghosh S; Division of Plant Biology, Bose Institute, Centenary Campus P-1/12 C.I.T., Scheme-VIIM Kolkata, 700054, West Bengal, India.
  • Saha U; Division of Plant Biology, Bose Institute, Centenary Campus P-1/12 C.I.T., Scheme-VIIM Kolkata, 700054, West Bengal, India.
  • Bose R; Division of Plant Biology, Bose Institute, Centenary Campus P-1/12 C.I.T., Scheme-VIIM Kolkata, 700054, West Bengal, India.
  • Chatterjee S; Department of Botany, Sister Nivedita Government General Degree College for Girls, 20B Judge's Court Road, Hastings House, Alipore, Kolkata, 700027, West Bengal, India.
  • Dey N; Department of Genetics, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, West Bengal, India.
  • Basu D; Division of Plant Biology, Bose Institute, Centenary Campus P-1/12 C.I.T., Scheme-VIIM Kolkata, 700054, West Bengal, India.
Mol Plant Microbe Interact ; 32(10): 1429-1447, 2019 Oct.
Article en En | MEDLINE | ID: mdl-31184524
Concomitant increase of auxin-responsive factors ARF16 and ARF17, along with enhanced expression of ARF10 in resistant Sinapis alba compared with that in susceptible Brassica juncea upon challenge with Alternaria brassicicola, revealed that abscisic acid (ABA)-auxin crosstalk is a critical factor for resistance response. Here, we induced the ABA response through conditional expression of ARF10 in B. juncea using the A. brassicicola-inducible GH3.3 promoter. Induced ABA sensitivity caused by conditional expression of ARF10 in transgenic B. juncea resulted in tolerance against A. brassicicola and led to enhanced expression of several ABA-responsive genes without affecting the auxin biosynthetic gene expression. Compared with ABI3 and ABI4, ABI5 showed maximum upregulation in the most tolerant transgenic lines upon pathogen challenge. Moreover, elevated expression of ARF10 by different means revealed a direct correlation between ARF10 expression and the induction of ABI5 protein in B. juncea. Through in vitro DNA-protein experiments and chromosome immunoprecipitation using the ARF10 antibody, we demonstrated that ARF10 interacts with the auxin-responsive elements of the ABI5 promoter. This suggests that ARF10 may function as a modulator of ABI5 to induce ABA sensitivity and mediate the resistance response against A. brassicicola.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Ácido Abscísico / Regulación de la Expresión Génica de las Plantas / Proteínas de Arabidopsis / Alternaria / Planta de la Mostaza Tipo de estudio: Diagnostic_studies Idioma: En Revista: Mol Plant Microbe Interact Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA / MICROBIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Ácido Abscísico / Regulación de la Expresión Génica de las Plantas / Proteínas de Arabidopsis / Alternaria / Planta de la Mostaza Tipo de estudio: Diagnostic_studies Idioma: En Revista: Mol Plant Microbe Interact Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA / MICROBIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos