Binding of proteins, including pp60src, to activated CH-sepharose 4B.

Pavloff, N; Biquard, J M; Mariller, M; Rabotti, G C; Fossar, N; Gay, F; Semmel, M

Pavloff, N; Biquard, J M; Mariller, M; Rabotti, G C; Fossar, N; Gay, F; Semmel, M.

Afiliación

Pavloff N; Groupe 8 du C.N.R.S., Institut Gustave-Roussy, Villejuif, France.

Mol Biol Rep ; 12(2): 127-31, 1987.

Article en En | MEDLINE | ID: mdl-3118186

RESUMEN

Activated CH-Sepharose 4B and protein A Sepharose CL-4B can bind, selectively and non-specifically, polypeptides from chick embryo cells. The major polypeptides bound have apparent molecular masses of 57-60 kDa and 47-49 kDa and cannot be eluted by extensive washing with buffers containing detergents. One of the 57-60 kDa polypeptides was identified by immunoblotting as the transforming protein of Rous Sarcoma Virus (RSV), pp60src. This polypeptide could be removed from the solid phase immunoabsorbent with 60% dimethylsulfoxide, but not with 2% SDS, 5% beta-mercaptoethanol, 1 M NaCl or 0.1% Tween 20.

Asunto(s)

Unión Proteica; Sefarosa/metabolismo; Animales; Embrión de Pollo; Proteína Oncogénica pp60(v-src); Conejos; Proteínas de los Retroviridae/metabolismo; Sefarosa/análogos & derivados; Proteína Estafilocócica A/metabolismo

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Unión Proteica / Sefarosa Límite: Animals Idioma: En Revista: Mol Biol Rep Año: 1987 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Países Bajos

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links