Your browser doesn't support javascript.
loading
Gene expression analyses in malformed skeletal structures of gilthead sea bream (Sparus aurata).
Riera-Heredia, Natàlia; Vélez, Emilio J; Gutiérrez, Joaquim; Navarro, Isabel; Capilla, Encarnación.
Afiliación
  • Riera-Heredia N; Departament de Biologia Cel·lular, Fisiologia i Immunologia, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain.
  • Vélez EJ; Departament de Biologia Cel·lular, Fisiologia i Immunologia, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain.
  • Gutiérrez J; Departament de Biologia Cel·lular, Fisiologia i Immunologia, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain.
  • Navarro I; Departament de Biologia Cel·lular, Fisiologia i Immunologia, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain.
  • Capilla E; Departament de Biologia Cel·lular, Fisiologia i Immunologia, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain.
J Fish Dis ; 42(8): 1169-1180, 2019 Aug.
Article en En | MEDLINE | ID: mdl-31180144
The incidence of skeletal anomalies in reared fish has been translated for years in important economic losses for the aquaculture industry. In the present study, we have analysed the gene expression of extracellular matrix components and transcription factors involved in bone development in gilthead sea bream presenting different skeletal anomalies: lordosis (LD), lordosis-scoliosis-kyphosis (LSK) or opercular, dental or jaw malformations in comparison with control (CT) specimens. Results showed a possible link between the presence of LD and LSK and the significant downregulation of genes involved in osteoblasts' maturation and matrix mineralization (collagen type 1-alpha, osteopontin, osteocalcin, matrix Gla protein and tissue non-specific alkaline phosphatase), as well as in bone resorption (cathepsin K and matrix metalloproteinase 9) compared to CT animals. Contrarily, the key osteogenic transcription factor runx2 was upregulated in the malformed vertebra suggesting impaired determination of mesenchymal stem cells towards the osteoblastic lineage. Despite the gene expression patterns of the other malformed structures were not affected in comparison with CT fish, the results of the present study may contribute in the long term to identify potential candidate gene profiles associated with column deformities that may help reducing the incidence of appearance of skeletal anomalies in this important aquaculture species.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Expresión Génica / Dorada / Matriz Extracelular / Enfermedades de los Peces / Anomalías Musculoesqueléticas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Fish Dis Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2019 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Expresión Génica / Dorada / Matriz Extracelular / Enfermedades de los Peces / Anomalías Musculoesqueléticas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Fish Dis Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2019 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido