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CgGCS, Encoding a Glucosylceramide Synthase, Is Required for Growth, Conidiation and Pathogenicity in Colletotrichum gloeosporioides.
Huang, Yimei; Li, Bin; Yin, Jian; Yang, Qiaosong; Sheng, Ou; Deng, Guiming; Li, Chunyu; Hu, Chunhua; Dong, Tao; Dou, Tongxin; Gao, Huijun; Bi, Fangcheng; Yi, Ganjun.
Afiliación
  • Huang Y; Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
  • Li B; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China.
  • Yin J; Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Province, Guangzhou, China.
  • Yang Q; College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China.
  • Sheng O; Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
  • Deng G; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China.
  • Li C; Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Province, Guangzhou, China.
  • Hu C; College of Life Sciences, South China Agricultural University, Guangzhou, China.
  • Dong T; School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
  • Dou T; Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
  • Gao H; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, China.
  • Bi F; Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Province, Guangzhou, China.
  • Yi G; Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Front Microbiol ; 10: 1016, 2019.
Article en En | MEDLINE | ID: mdl-31164871
Fungal glucosylceramide plays important role in cell division, hyphal formation and growth, spore germination and the modulation of virulence and has recently been considered as target for small molecule inhibitors. In this study, we characterized CgGCS, a protein encoding a glucosylceramide synthase (GCS) in Colletotrichum gloeosporioides. Disruption of CgGCS resulted in a severe reduction of mycelial growth and defects in conidiogenesis. Sphingolipid profile analysis revealed large decreases in glucosylceramide production in the mutant strains. Pathogenicity assays indicated that the ability of the ΔCgGCS mutants to invade both tomato and mango hosts was almost lost. In addition, the expression levels of many genes, especially those related to metabolism, were shown to be affected by the mutation of CgGCS via transcriptome analysis. Overall, our results demonstrate that C. gloeosporioides glucosylceramide is an important regulatory factor in fungal growth, conidiation, and pathogenesis in hosts.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Microbiol Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Microbiol Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza