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Wolbachia pipientis occurs in Aedes aegypti populations in New Mexico and Florida, USA.
Kulkarni, Aditi; Yu, Wanqin; Jiang, Jinjin; Sanchez, Concepcion; Karna, Ajit K; Martinez, Kalli J L; Hanley, Kathryn A; Buenemann, Michaela; Hansen, Immo A; Xue, Rui-de; Ettestad, Paul; Melman, Sandra; Duguma, Dagne; Debboun, Mustapha; Xu, Jiannong.
Afiliación
  • Kulkarni A; Biology Department New Mexico State University Las Cruces New Mexico.
  • Yu W; Biology Department New Mexico State University Las Cruces New Mexico.
  • Jiang J; Biology Department New Mexico State University Las Cruces New Mexico.
  • Sanchez C; Biology Department New Mexico State University Las Cruces New Mexico.
  • Karna AK; Biology Department New Mexico State University Las Cruces New Mexico.
  • Martinez KJL; Biology Department New Mexico State University Las Cruces New Mexico.
  • Hanley KA; Biology Department New Mexico State University Las Cruces New Mexico.
  • Buenemann M; Department of Geography New Mexico State University Las Cruces New Mexico.
  • Hansen IA; Biology Department New Mexico State University Las Cruces New Mexico.
  • Xue RD; Anastasia Mosquito Control District St. Augustine Florida.
  • Ettestad P; New Mexico Department of Health Santa Fe New Mexico.
  • Melman S; New Mexico Department of Health Santa Fe New Mexico.
  • Duguma D; Harris County Public Health Mosquito and Vector Control Division Houston Texas.
  • Debboun M; Harris County Public Health Mosquito and Vector Control Division Houston Texas.
  • Xu J; Biology Department New Mexico State University Las Cruces New Mexico.
Ecol Evol ; 9(10): 6148-6156, 2019 May.
Article en En | MEDLINE | ID: mdl-31161026
The mosquitoes Aedes aegypti (L.) and Ae. albopictus Skuse are the major vectors of dengue, Zika, yellow fever, and chikungunya viruses worldwide. Wolbachia, an endosymbiotic bacterium present in many insects, is being utilized in novel vector control strategies to manipulate mosquito life history and vector competence to curb virus transmission. Earlier studies have found that Wolbachia is commonly detected in Ae. albopictus but rarely detected in Ae. aegypti. In this study, we used a two-step PCR assay to detect Wolbachia in wild-collected samples of Ae. aegypti. The PCR products were sequenced to validate amplicons and identify Wolbachia strains. A loop-mediated isothermal amplification (LAMP) assay was developed and used for detecting Wolbachia in selected mosquito specimens as well. We found Wolbachia in 85/148 (57.4%) wild Ae. aegypti specimens from various cities in New Mexico, and in 2/46 (4.3%) from St. Augustine, Florida. Wolbachia was not detected in 94 samples of Ae. aegypti from Deer Park, Harris County, Texas. Wolbachia detected in Ae. aegypti from both New Mexico and Florida was the wAlbB strain of Wolbachia pipientis. A Wolbachia-positive colony of Ae. aegypti was established from pupae collected in Las Cruces, New Mexico, in 2018. The infected females of this strain transmitted Wolbachia to their progeny when crossed with males of Rockefeller strain of Ae. aegypti, which does not carry Wolbachia. In contrast, none of the progeny of Las Cruces males mated to Rockefeller females were infected with Wolbachia.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies País/Región como asunto: Mexico Idioma: En Revista: Ecol Evol Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies País/Región como asunto: Mexico Idioma: En Revista: Ecol Evol Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido