CRISPRi as an efficient tool for gene repression in archaea.
Methods
; 172: 76-85, 2020 02 01.
Article
en En
| MEDLINE
| ID: mdl-31150759
In the years following its discovery and characterization, the CRISPR-Cas system has been modified and converted into a multitude of applications for eukaryotes and bacteria, such as genome editing and gene regulation. Since no such method has been available for archaea, we developed a tool for gene repression in the haloarchaeon Haloferax volcanii by repurposing its endogenous type I-B CRISPR-Cas system. Here, we present the two possible approaches for gene repression as well as our workflow to achieve and assess gene knockdown, offer recommendations on protospacer selection and give some examples of genes we have successfully silenced.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Haloferax volcanii
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Regulación de la Expresión Génica Arqueal
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Sistemas CRISPR-Cas
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Edición Génica
Idioma:
En
Revista:
Methods
Asunto de la revista:
BIOQUIMICA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Estados Unidos