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Quantification of Lipid Content in Oleaginous Biomass Using Thermogravimetry.
Maddi, Balakrishna; Vadlamani, Agasteswar; Viamajala, Sridhar; Varanasi, Sasidhar.
Afiliación
  • Maddi B; Suganit Bio-Renewables LLC, Toledo, OH, USA.
  • Vadlamani A; Department of Chemical Engineering, The University of Toledo, Toledo, OH, USA.
  • Viamajala S; Department of Chemical Engineering, The University of Toledo, Toledo, OH, USA. Sridhar.viamajala@utoledo.edu.
  • Varanasi S; Department of Chemical Engineering, The University of Toledo, Toledo, OH, USA.
Methods Mol Biol ; 1995: 121-129, 2019.
Article en En | MEDLINE | ID: mdl-31148124
Laboratory analytical techniques employed for triglyceride quantification in oleaginous biomass (e.g., microalgae and oilseeds) involve multiple steps and typically require use of volatile organic solvents. Here we describe a single-step approach for measurement of triglycerides using thermogravimetry (TG). We have observed that triglycerides undergo complete volatilization over a narrow temperature interval of 370-450 °C, with negligible solid residue under inert atmosphere, whereas other constituents of oleaginous biomass (such as proteins and carbohydrates) primarily degrade below 350 °C. As a result, triglyceride content of biomass can be estimated using TG by determining the mass loss of the sample in the temperature interval of 370-450 °C.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Termogravimetría / Triglicéridos / Microalgas Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Imprimir
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Termogravimetría / Triglicéridos / Microalgas Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos