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Nicking enzyme-assisted signal-amplifiable Hg2+ detection using upconversion nanoparticles.
Vijayan, Anjaly N; Liu, Zhiming; Zhao, Haohan; Zhang, Peng.
Afiliación
  • Vijayan AN; Department of Chemistry, University of Cincinnati, OH, 45221, USA.
  • Liu Z; National Engineering Laboratory for Green Chemical Productions of Alcohols, Ethers and Esters, College of Chemistry & Chemical Engineering, Xiamen University, Xiamen, 361005, China.
  • Zhao H; Department of Chemistry, University of Cincinnati, OH, 45221, USA.
  • Zhang P; Department of Chemistry, University of Cincinnati, OH, 45221, USA. Electronic address: peng.zhang@uc.edu.
Anal Chim Acta ; 1072: 75-80, 2019 Sep 23.
Article en En | MEDLINE | ID: mdl-31146867
A highly specific and sensitive isothermal method for mercury detection using DNA-conjugated upconversion nanoparticles is reported. A single-stranded DNA containing thymine bases, used as the Hg2+-capturing element through the formation of thymine-Hg2+-thymine complex, is covalently attached to the NaYF4: Yb3+, Tm3+ nanoparticles. Luminescence resonance energy transfer takes place between the NaYF4: Yb3+, Tm3+ nanoparticles as donor and DNA-intercalating SYBR Green I as the acceptor upon excitation of 980 nm. The sensitivity and selectivity toward Hg2+ are enhanced using the nicking enzyme, Nt. Alwl, which leads to signal amplification. By monitoring the ratio of acceptor emission to a reference peak, the presence of Hg2+ ions are quantitatively determined with a lower detection limit of 0.14 nM, which is much lower than the US Environmental Protection Agency (EPA) limit of Hg2+ in drinking water.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Técnicas de Amplificación de Ácido Nucleico / Nanopartículas / Mercurio Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Chim Acta Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Técnicas de Amplificación de Ácido Nucleico / Nanopartículas / Mercurio Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Chim Acta Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos