Your browser doesn't support javascript.
loading
The state-of-the-art strategies of protein engineering for enzyme stabilization.
Liu, Qian; Xun, Guanhua; Feng, Yan.
Afiliación
  • Liu Q; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
  • Xun G; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
  • Feng Y; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: yfeng2009@sjtu.edu.cn.
Biotechnol Adv ; 37(4): 530-537, 2019.
Article en En | MEDLINE | ID: mdl-31138425
Enzymes generated by natural recruitment and protein engineering have greatly contribute in various sets of applications. However, their insufficient stability is a bottleneck that limit the rapid development of biocatalysis. Novel approaches based on precise and global structural dissection, advanced gene manipulation, and combination with the multidisciplinary techniques open a new horizon to generate stable enzymes efficiently. Here, we comprehensively introduced emerging advances of protein engineering strategies for enzyme stabilization. Then, we highlighted practical cases to show importance of enzyme stabilization in pharmaceutical and industrial applications. Combining computational enzyme design with molecular evolution will hold considerable promise in this field.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biotecnología / Estabilidad de Enzimas / Ingeniería de Proteínas / Enzimas Idioma: En Revista: Biotechnol Adv Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biotecnología / Estabilidad de Enzimas / Ingeniería de Proteínas / Enzimas Idioma: En Revista: Biotechnol Adv Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido