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Preparation of a novel polyethyleneimine functionalized sepharose-boronate affinity material and its application in selective enrichment of food borne pathogenic bacteria.
Zheng, Hongwei; Han, Feng; Lin, Hong; Cao, Limin; Pavase, Tushar Ramesh; Sui, Jianxin.
Afiliación
  • Zheng H; College of Food Science and Engineering, Food Safety Laboratory, Ocean University of China, Qingdao 266003, People's Republic of China.
  • Han F; East China Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Shanghai 200090, People's Republic of China.
  • Lin H; College of Food Science and Engineering, Food Safety Laboratory, Ocean University of China, Qingdao 266003, People's Republic of China.
  • Cao L; College of Food Science and Engineering, Food Safety Laboratory, Ocean University of China, Qingdao 266003, People's Republic of China.
  • Pavase TR; College of Food Science and Engineering, Food Safety Laboratory, Ocean University of China, Qingdao 266003, People's Republic of China.
  • Sui J; College of Food Science and Engineering, Food Safety Laboratory, Ocean University of China, Qingdao 266003, People's Republic of China. Electronic address: suijianxin@ouc.edu.cn.
Food Chem ; 294: 468-476, 2019 Oct 01.
Article en En | MEDLINE | ID: mdl-31126489
Detection of pathogenic bacteria by polymerase chain reaction (PCR) is progressively emerging, although it is still hindered by a complex matrix, long-term bacterial enrichment and low bacterial abundance. Here, we report a novel material based on boronate affinity for recognition and enrichment of bacteria using a pre-PCR method. After optimization, the material exhibited high boronate affinity toward bacteria, with adsorption capacities of S. aureus and Salmonella spp. incubated in 0.01 M PBS (pH 7.4) at 37 °C for 15 min calculated as (906.60 ±â€¯15.73) × 107 cfu/g and (582.59 ±â€¯13.19) × 107 cfu/g, respectively, without any bacterial death during the binding process. The material was then applied to enrich S. aureus and Salmonella spp. from the spiked water and 25% cow milk samples followed by mPCR, which resulted in high bacterial enrichment and demonstrated great potential for selective enrichment of bacteria in food samples.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Salmonella / Staphylococcus aureus / Compuestos de Boro / Microbiología de Alimentos Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Food Chem Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Salmonella / Staphylococcus aureus / Compuestos de Boro / Microbiología de Alimentos Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Food Chem Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido