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Resveratrol-delivery vehicle with anti-VEGF activity carried to human retinal pigmented epithelial cells exposed to high-glucose induced conditions.
Ruginǎ, Dumitrita; Ghiman, Raluca; Focșan, Monica; Tabaran, Flaviu; Copaciu, Florina; Suciu, Maria; Pintea, Adela; Aștilean, Simion.
Afiliación
  • Ruginǎ D; Biochemistry Department, Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine, Calea Manastur, No. 3-5, Cluj-Napoca 400372, Romania.
  • Ghiman R; Nanobiophotonics and Laser Microspectroscopy Center, Interdisciplinary Research Institute on Bio-Nano-Sciences, Babes-Bolyai University, Treboniu Laurean No. 42, Cluj-Napoca 400271, Romania.
  • Focșan M; Nanobiophotonics and Laser Microspectroscopy Center, Interdisciplinary Research Institute on Bio-Nano-Sciences, Babes-Bolyai University, Treboniu Laurean No. 42, Cluj-Napoca 400271, Romania.
  • Tabaran F; Biochemistry Department, Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine, Calea Manastur, No. 3-5, Cluj-Napoca 400372, Romania.
  • Copaciu F; Biochemistry Department, Faculty of Animal Sciences and Biotechnologies, Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine, Calea Manastur, No. 3-5, Cluj-Napoca 400372, Romania.
  • Suciu M; National Institute for Research and Development of Isotopic and Molecular Technologies, Donath No. 67-103, Cluj-Napoca 400293, Romania; Biology and Geology Faculty, Babes-Bolyai University Cluj-Napoca, Clinicilor, No. 5-7, Cluj-Napoca 400006, Romania.
  • Pintea A; Biochemistry Department, Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine, Calea Manastur, No. 3-5, Cluj-Napoca 400372, Romania. Electronic address: apintea@usamvcluj.ro.
  • Aștilean S; Nanobiophotonics and Laser Microspectroscopy Center, Interdisciplinary Research Institute on Bio-Nano-Sciences, Babes-Bolyai University, Treboniu Laurean No. 42, Cluj-Napoca 400271, Romania. Electronic address: simion.astilean@phys.ubbcluj.ro.
Colloids Surf B Biointerfaces ; 181: 66-75, 2019 Sep 01.
Article en En | MEDLINE | ID: mdl-31125919
As an integrated approach to defeat diabetic retinopathy, a common complication of diabetes leading to vision loss, a delivery vehicle able to transport resveratrol (Rv) directly into retina pigmented epithelial D407 cells was designed. Rv, a molecule with known therapeutic potential, was successfully inserted into a microcapsule based on porous CaCO3 templates revealing an encapsulation efficiency of 96.8 ±â€¯4.0%. Four alternative layers of polyelectrolytes were deposited via electrostatic-driven layer-by-layer assembly approach on the template and covered by rhodamine 6G (Rh6G). The as-designed PMs-Rv-Rh6G microcapsules were internalized into D407 cells grown in normal and high glucose-induced inflammation conditions, being able to cross the cellular membrane and localize near the nucleus after 24 h treatment. The metabolic activity of D407 cells was not diminished by PMs-Rv-Rh6G even after 24 h treatment, meaning that the microcapsules do not exert any toxicity toward the cells, based on WST-1 and lactate dehydrogenase assays. Notably, the PMs-Rv-Rh6G treatment is able to inhibit the vascular endothelial growth factor (VEGF) protein, as was proved by the ELISA assay. Therefore, the proposed PMs-Rv-Rh6G microcapsules could be implemented as a potential self-reporting intraocular Rv-delivery vehicle with anti-VEGF activity in the management of diabetic retinopathy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sistemas de Liberación de Medicamentos / Inhibidores de la Angiogénesis / Factor A de Crecimiento Endotelial Vascular / Epitelio Pigmentado de la Retina / Resveratrol / Glucosa Límite: Humans Idioma: En Revista: Colloids Surf B Biointerfaces Asunto de la revista: QUIMICA Año: 2019 Tipo del documento: Article País de afiliación: Rumanía Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sistemas de Liberación de Medicamentos / Inhibidores de la Angiogénesis / Factor A de Crecimiento Endotelial Vascular / Epitelio Pigmentado de la Retina / Resveratrol / Glucosa Límite: Humans Idioma: En Revista: Colloids Surf B Biointerfaces Asunto de la revista: QUIMICA Año: 2019 Tipo del documento: Article País de afiliación: Rumanía Pais de publicación: Países Bajos