Your browser doesn't support javascript.
loading
A Fast and Reliable Method to Isolate Human Placental Macrophages.
Mezouar, Soraya; Ben Amara, Amira; Chartier, Céline; Gorvel, Laurent; Mege, Jean-Louis.
Afiliación
  • Mezouar S; Aix-Marseille University, IRD, APHM, MEPHI, IHU-Méditerranée Infection, Marseille, France.
  • Ben Amara A; CRCM, CNRS UMR7258, INSERM U1068, Institut Paoli-Calmettes, Aix-Marseille University, Marseille, France.
  • Chartier C; Aix-Marseille University, IRD, APHM, MEPHI, IHU-Méditerranée Infection, Marseille, France.
  • Gorvel L; CRCM, CNRS UMR7258, INSERM U1068, Institut Paoli-Calmettes, Aix-Marseille University, Marseille, France.
  • Mege JL; Aix-Marseille University, IRD, APHM, MEPHI, IHU-Méditerranée Infection, Marseille, France.
Curr Protoc Immunol ; 125(1): e77, 2019 06.
Article en En | MEDLINE | ID: mdl-31125190
Macrophages are specialized cells involved in recognition, uptake, and destruction of microorganisms. Human placental macrophages are poorly investigated because of the lack of a convenient protocol for their isolation. Here, we present a straightforward and reliable method to isolate macrophages from full-term human placentas. After enzymatic digestion of placental tissue and centrifugation of the cell suspension on a Ficoll cushion, placental macrophages are selected using magnetic beads coated with anti-CD14 antibodies. Isolated cells are characterized by flow cytometry. Ninety eight percent of isolated CD14+ placental macrophages also express the macrophage marker CD68. Thus, this efficient and reliable method yields placental macrophages at high purity and sufficient quantity for functional studies. © 2019 by John Wiley & Sons, Inc.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Separación Celular / Macrófagos Límite: Female / Humans / Pregnancy Idioma: En Revista: Curr Protoc Immunol Año: 2019 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Separación Celular / Macrófagos Límite: Female / Humans / Pregnancy Idioma: En Revista: Curr Protoc Immunol Año: 2019 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos