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Polymer-Coated Gold Nanospheres Do Not Impair the Innate Immune Function of Human B Lymphocytes in Vitro.
Hocevar, Sandra; Milosevic, Ana; Rodriguez-Lorenzo, Laura; Ackermann-Hirschi, Liliane; Mottas, Ines; Petri-Fink, Alke; Rothen-Rutishauser, Barbara; Bourquin, Carole; Clift, Martin James David.
Afiliación
  • Hocevar S; BioNanomaterials , Adolphe Merkle Institute, University of Fribourg , 1700 Fribourg , Switzerland.
  • Milosevic A; School of Pharmaceutical Sciences , University of Geneva, University of Lausanne , 1211 Geneva , Switzerland.
  • Rodriguez-Lorenzo L; BioNanomaterials , Adolphe Merkle Institute, University of Fribourg , 1700 Fribourg , Switzerland.
  • Ackermann-Hirschi L; BioNanomaterials , Adolphe Merkle Institute, University of Fribourg , 1700 Fribourg , Switzerland.
  • Mottas I; BioNanomaterials , Adolphe Merkle Institute, University of Fribourg , 1700 Fribourg , Switzerland.
  • Petri-Fink A; School of Pharmaceutical Sciences , University of Geneva, University of Lausanne , 1211 Geneva , Switzerland.
  • Rothen-Rutishauser B; Chair of Pharmacology, Faculty of Science and Medicine , University of Fribourg , 1700 Fribourg , Switzerland.
  • Bourquin C; BioNanomaterials , Adolphe Merkle Institute, University of Fribourg , 1700 Fribourg , Switzerland.
  • Clift MJD; BioNanomaterials , Adolphe Merkle Institute, University of Fribourg , 1700 Fribourg , Switzerland.
ACS Nano ; 13(6): 6790-6800, 2019 06 25.
Article en En | MEDLINE | ID: mdl-31117377
Gold nanoparticles (GNPs) are intended for use within a variety of biomedical applications due to their physicochemical properties. Although, in general, biocompatibility of GNPs with immune cells such as macrophages and dendritic cells is well established, the impact of GNPs on B lymphocyte immune function remains to be determined. Since B lymphocytes play an important role in health and disease, the suitability of GNPs as a B cell-targeting tool is of high relevance. Thus, we provide information on the interactions of GNPs with B lymphocytes. Herein, we exposed freshly isolated human B lymphocytes to a set of well-characterized and biomedically relevant GNPs with distinct surface (polyethylene glycol (PEG), PEG/poly(vinyl alcohol) (PEG/PVA)) and shape (spheres, rods) characteristics. Polymer-coated GNPs poorly interacted with B lymphocytes, in contrast to uncoated GNPs. Importantly, none of the GNPs significantly affected cell viability, even at the highest concentration of 20 µg/mL over a 24 h suspension exposure period. Furthermore, none of the nanosphere formulations affected the expression of activation markers (CD69, CD86, MHC II) of the naive B lymphocytes, nor did they cause an increase in the secretion of pro-inflammatory cytokines ( i.e. , IL-6, IL-1ß). However, the absence of polymer coating on the sphere GNPs and the rod shape caused a decrease in IL-6 cytokine production by activated B lymphocytes, suggesting a functional impairment. With these findings, the present study contributes imperative knowledge toward the safe-by-design approaches being conducted to benefit the development of nanomaterials, specifically those as theranostic tools.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos B / Nanosferas / Inmunidad Innata Límite: Humans Idioma: En Revista: ACS Nano Año: 2019 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos B / Nanosferas / Inmunidad Innata Límite: Humans Idioma: En Revista: ACS Nano Año: 2019 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Estados Unidos