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Differential Responses of Bovine Monocyte-Derived Macrophages to Infection by Neospora caninum Isolates of High and Low Virulence.
García-Sánchez, Marta; Jiménez-Pelayo, Laura; Horcajo, Pilar; Regidor-Cerrillo, Javier; Ólafsson, Einar B; Bhandage, Amol K; Barragan, Antonio; Werling, Dirk; Ortega-Mora, Luis Miguel; Collantes-Fernández, Esther.
Afiliación
  • García-Sánchez M; SALUVET, Animal Health Department, Complutense University of Madrid, Madrid, Spain.
  • Jiménez-Pelayo L; SALUVET, Animal Health Department, Complutense University of Madrid, Madrid, Spain.
  • Horcajo P; SALUVET, Animal Health Department, Complutense University of Madrid, Madrid, Spain.
  • Regidor-Cerrillo J; SALUVET, Animal Health Department, Complutense University of Madrid, Madrid, Spain.
  • Ólafsson EB; Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden.
  • Bhandage AK; Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden.
  • Barragan A; Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden.
  • Werling D; Department of Pathobiology and Population Sciences, Royal Veterinary College, University of London, North Mymms, United Kingdom.
  • Ortega-Mora LM; SALUVET, Animal Health Department, Complutense University of Madrid, Madrid, Spain.
  • Collantes-Fernández E; SALUVET, Animal Health Department, Complutense University of Madrid, Madrid, Spain.
Front Immunol ; 10: 915, 2019.
Article en En | MEDLINE | ID: mdl-31114577
Neospora caninum, a protozoan parasite closely related to Toxoplasma gondii, represents one of the main causes of abortion in cattle. Macrophages (MØs) are mediators of the innate immune response against infection and likely one of the first cells encountered by the parasite during the host infection process. In this study, we investigated in vitro how high or low virulent isolates of N. caninum (Nc-Spain7 and Nc-Spain1H, respectively) interact with bovine monocyte-derived MØs and the influence of the isolate virulence on the subsequent cellular response. Both isolates actively invaded, survived and replicated in the MØs. However, Nc-Spain7 showed a higher invasion rate and a replication significantly faster, following an exponential growth model, whereas Nc-Spain1H presented a delayed replication and a lower growth rate without an exponential pattern. N. caninum infection induced a hypermigratory phenotype in bovine MØs that was characterized by enhanced motility and transmigration in vitro and was accompanied by morphological changes and abrogated extracellular matrix degradation. A significantly higher hypermotility was observed with the highly virulent isolate Nc-Spain7. Nc-Spain1H-infected MØs showed elevated reactive oxygen species (ROS) production and IL12p40 expression, which also resulted in increased IFN-γ release by lymphocytes, compared to cells infected with Nc-Spain7. Furthermore, IL-10 was upregulated in MØs infected with both isolates. Infected MØs exhibited lower expression of MHC Class II, CD86, and CD1b molecules than uninfected MØs, with non-significant differences between isolates. This work characterizes for the first time N. caninum replication in bovine monocyte-derived MØs and details isolate-dependent differences in host cell responses to the parasite.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Monocitos / Enfermedades de los Bovinos / Coccidiosis / Neospora / Macrófagos Límite: Animals Idioma: En Revista: Front Immunol Año: 2019 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Monocitos / Enfermedades de los Bovinos / Coccidiosis / Neospora / Macrófagos Límite: Animals Idioma: En Revista: Front Immunol Año: 2019 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza