Modulating PKCα Activity to Target Wnt/ß-Catenin Signaling in Colon Cancer.
Cancers (Basel)
; 11(5)2019 May 18.
Article
en En
| MEDLINE
| ID: mdl-31109112
Inactivating mutations of the tumor suppressor Adenomatosis Polyposis Coli (APC), which are found in familial adenomatosis polyposis and in 80% of sporadic colorectal cancers (CRC), result in constitutive activation of the Wnt/ß-catenin pathway and tumor development in the intestine. These mutations disconnect the Wnt/ß-catenin pathway from its Wnt extracellular signal by inactivating the APC/GSK3-ß/axin destruction complex of ß-catenin. This results in sustained nuclear accumulation of ß-catenin, followed by ß-catenin-dependent co-transcriptional activation of Wnt/ß-catenin target genes. Thus, mechanisms acting downstream of APC, such as those controlling ß-catenin stability and/or co-transcriptional activity, are attractive targets for CRC treatment. Protein Kinase C-α (PKCα) phosphorylates the orphan receptor RORα that then inhibits ß-catenin co-transcriptional activity. PKCα also phosphorylates ß-catenin, leading to its degradation by the proteasome. Here, using both in vitro (DLD-1 cells) and in vivo (C57BL/6J mice) PKCα knock-in models, we investigated whether enhancing PKCα function could be beneficial in CRC treatment. We found that PKCα is infrequently mutated in CRC samples, and that inducing PKCα function is not deleterious for the normal intestinal epithelium. Conversely, di-terpene ester-induced PKCα activity triggers CRC cell death. Together, these data indicate that PKCα is a relevant drug target for CRC treatment.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Cancers (Basel)
Año:
2019
Tipo del documento:
Article
País de afiliación:
Bélgica
Pais de publicación:
Suiza