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Lightsheet localization microscopy enables fast, large-scale, and three-dimensional super-resolution imaging.
Lu, Chieh-Han; Tang, Wei-Chun; Liu, Yen-Ting; Chang, Shu-Wei; Wu, Frances Camille M; Chen, Chin-Yi; Tsai, Yun-Chi; Yang, Shun-Min; Kuo, Chiung-Wen; Okada, Yasushi; Hwu, Yeu-Kuang; Chen, Peilin; Chen, Bi-Chang.
Afiliación
  • Lu CH; 1Research Center for Applied Sciences, Academia Sinica, Taipei, 11529 Taiwan.
  • Tang WC; 1Research Center for Applied Sciences, Academia Sinica, Taipei, 11529 Taiwan.
  • Liu YT; 1Research Center for Applied Sciences, Academia Sinica, Taipei, 11529 Taiwan.
  • Chang SW; 1Research Center for Applied Sciences, Academia Sinica, Taipei, 11529 Taiwan.
  • Wu FCM; 1Research Center for Applied Sciences, Academia Sinica, Taipei, 11529 Taiwan.
  • Chen CY; 1Research Center for Applied Sciences, Academia Sinica, Taipei, 11529 Taiwan.
  • Tsai YC; 1Research Center for Applied Sciences, Academia Sinica, Taipei, 11529 Taiwan.
  • Yang SM; 2Institute of Physics, Academia Sinica, Taipei, 11529 Taiwan.
  • Kuo CW; 1Research Center for Applied Sciences, Academia Sinica, Taipei, 11529 Taiwan.
  • Okada Y; 3Laboratory for Cell Polarity Regulation, Center for Biosystems Dynamics Research, RIKEN, Suita, Osaka, 565-0874 Japan.
  • Hwu YK; 4Department of Physics, Universal Biology Institute and International Research Center for Neurointelligence, The University of Tokyo, Tokyo, 113-0033 Japan.
  • Chen P; 2Institute of Physics, Academia Sinica, Taipei, 11529 Taiwan.
  • Chen BC; 1Research Center for Applied Sciences, Academia Sinica, Taipei, 11529 Taiwan.
Commun Biol ; 2: 177, 2019.
Article en En | MEDLINE | ID: mdl-31098410
Recent advances in super-resolution microscopy allow the localization of single molecules within individual cells but not within multiple whole cells due to weak signals from single molecules and slow acquisition process for point accumulation to reconstruct super-resolution images. Here, we report a fast, large-scale, and three-dimensional super-resolution fluorescence microscope based on single-wavelength Bessel lightsheet to selectively illuminate spontaneous blinking fluorophores tagged to the proteins of interest in space. Critical parameters such as labeling density, excitation power, and exposure time were systematically optimized resulting in a maximum imaging speed of 2.7 × 104 µm3 s-1. Fourier ring correlation analysis revealed a reconstructed image with a lateral resolution of ~75 nm through the accumulation of 250 image volumes on immobilized samples within 15 min. Hence, the designed system could open new insights into the discovery of complex biological structures and live 3D localization imaging.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Imagenología Tridimensional / Microscopía Fluorescente Límite: Animals Idioma: En Revista: Commun Biol Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Imagenología Tridimensional / Microscopía Fluorescente Límite: Animals Idioma: En Revista: Commun Biol Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido