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Storage mite concentrations are underestimated compared to house dust mite concentrations.
Reboux, Gabriel; Valot, Benoit; Rocchi, Steffi; Scherer, Emeline; Roussel, Sandrine; Millon, Laurence.
Afiliación
  • Reboux G; UMR/CNRS 6249, Chrono-Environnement, University of Bourgogne-Franche-Comté, Besançon, France. gabriel.reboux@univ-fcomte.fr.
  • Valot B; Department of Parasitology-Mycology, UMR CNRS 6249, University Hospital of Besançon, Bd Fleming, 25030, Besançon, France. gabriel.reboux@univ-fcomte.fr.
  • Rocchi S; UMR/CNRS 6249, Chrono-Environnement, University of Bourgogne-Franche-Comté, Besançon, France.
  • Scherer E; UMR/CNRS 6249, Chrono-Environnement, University of Bourgogne-Franche-Comté, Besançon, France.
  • Roussel S; Department of Parasitology-Mycology, UMR CNRS 6249, University Hospital of Besançon, Bd Fleming, 25030, Besançon, France.
  • Millon L; UMR/CNRS 6249, Chrono-Environnement, University of Bourgogne-Franche-Comté, Besançon, France.
Exp Appl Acarol ; 77(4): 511-525, 2019 Apr.
Article en En | MEDLINE | ID: mdl-31093854
Dwellings are increasingly well insulated to save energy and this leads to higher humidity and temperature, which improves conditions for mites. Dermatophagoides antigens are the main allergens involved and tested in atopic asthma. We developed three new species-specific quantitative PCR (qPCR) methods for house dust mites (Dermatophagoides pteronyssinus and D. farinae) and storages mites (Acarus siro, Glycyphagus domesticus, Lepidoglyphus destructor). We sampled dust with electrostatic dust collectors, in the bedrooms, under beds and in the kitchens of patients with allergies (n = 24) and healthy controls (n = 18). Mite quantification was carried out with the three new qPCRs and the qPCR previously described for the Dermatophagoides genus. The qPCRs were highly specific and efficient for house dust mite species and the storage mites. Storage mite concentrations were higher than house dust mite concentrations and were higher in dwellings of patients with allergies. Consequently, allergists should test more often patients against the storage mite antigens by prick tests or IgE serology. Dampness is a major factor in storage mite development and the presence of effective mechanical ventilation can reduce storage mite concentrations four-fold. In addition, to limit exposure to dust mites, treatments should be used throughout dwellings and not only in patients' bedrooms.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reacción en Cadena en Tiempo Real de la Polimerasa / Distribución Animal / Vivienda / Ácaros Tipo de estudio: Etiology_studies Aspecto: Determinantes_sociais_saude Límite: Animals / Humans País/Región como asunto: Europa Idioma: En Revista: Exp Appl Acarol Asunto de la revista: BIOLOGIA / PARASITOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reacción en Cadena en Tiempo Real de la Polimerasa / Distribución Animal / Vivienda / Ácaros Tipo de estudio: Etiology_studies Aspecto: Determinantes_sociais_saude Límite: Animals / Humans País/Región como asunto: Europa Idioma: En Revista: Exp Appl Acarol Asunto de la revista: BIOLOGIA / PARASITOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Países Bajos