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Direct determination of anaerobe contributions to the energy metabolism of Trypanosoma cruzi by chip calorimetry.
Lerchner, Johannes; Sartori, Marina R; Volpe, Pedro O L; Lander, Noelia; Mertens, Florian; Vercesi, Anibal E.
Afiliación
  • Lerchner J; Institute of Physical Chemistry, TU Bergakademie Freiberg, Leipziger Str. 29, 09599, Freiberg, Germany. lerchne1@mailserver.tu-freiberg.de.
  • Sartori MR; Department of Clinical Pathology, University of Campinas (UNICAMP), Campinas, SP, 13083-877, Brazil.
  • Volpe POL; Institute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, 13083-970, Brazil.
  • Lander N; Department of Clinical Pathology, University of Campinas (UNICAMP), Campinas, SP, 13083-877, Brazil.
  • Mertens F; Institute of Physical Chemistry, TU Bergakademie Freiberg, Leipziger Str. 29, 09599, Freiberg, Germany.
  • Vercesi AE; Department of Clinical Pathology, University of Campinas (UNICAMP), Campinas, SP, 13083-877, Brazil.
Anal Bioanal Chem ; 411(17): 3763-3768, 2019 Jul.
Article en En | MEDLINE | ID: mdl-31093698
We describe a chip calorimetric technique that allows the investigation of biological material under anoxic conditions in a micro-scale and in real time. Due to the fast oxygen exchange through the sample flow channel wall, the oxygen concentration inside the samples could be switched between atmospheric oxygen partial pressure to an oxygen concentration of 0.5% within less than 2 h. Using this technique, anaerobic processes in the energy metabolism of Trypanosoma cruzi could be studied directly. The comparison of the calorimetric and respirometric response of T. cruzi cells to the treatment with the mitochondrial inhibitors oligomycin and antimycin A and the uncoupler FCCP revealed that the respiration-related heat rate is superimposed by strong anaerobic contributions. Calorimetric measurements under anoxic conditions and with glycolytic inhibitors showed that anaerobic metabolic processes contribute from 30 to 40% to the overall heat production rate. Similar basal and antimycin A heat rates with cells under anoxic conditions indicated that the glycolytic rates are independent of the oxygen concentration which confirms the absence of the "Pasteur effect" in Trypanosomes. Graphical abstract.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trypanosoma cruzi / Calorimetría / Metabolismo Energético / Dispositivos Laboratorio en un Chip Idioma: En Revista: Anal Bioanal Chem Año: 2019 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trypanosoma cruzi / Calorimetría / Metabolismo Energético / Dispositivos Laboratorio en un Chip Idioma: En Revista: Anal Bioanal Chem Año: 2019 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania