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Relevance of anti-HNK1 antibodies in the management of anti-MAG neuropathies.
Delmont, Emilien; Attarian, Shahram; Antoine, Jean-Christophe; Paul, Stephane; Camdessanché, Jean Philippe; Grapperon, Aude-Marie; Brodovich, Alexandre; Boucraut, Jose.
Afiliación
  • Delmont E; Referral Centre for ALS and Neuromuscular Diseases, Hospital La Timone 264 rue Saint Pierre, 13005, Marseille, France. emilien.delmont@ap-hm.fr.
  • Attarian S; Referral Centre for ALS and Neuromuscular Diseases, Hospital La Timone 264 rue Saint Pierre, 13005, Marseille, France.
  • Antoine JC; Department of Neurology and Referral Centre for Neuromuscular Diseases, Saint Etienne, France.
  • Paul S; Immunology Laboratory, Saint Etienne, France.
  • Camdessanché JP; Department of Neurology and Referral Centre for Neuromuscular Diseases, Saint Etienne, France.
  • Grapperon AM; Referral Centre for ALS and Neuromuscular Diseases, Hospital La Timone 264 rue Saint Pierre, 13005, Marseille, France.
  • Brodovich A; Referral Centre for ALS and Neuromuscular Diseases, Hospital La Timone 264 rue Saint Pierre, 13005, Marseille, France.
  • Boucraut J; Immunology Laboratory, Marseille, France.
J Neurol ; 266(8): 1973-1979, 2019 Aug.
Article en En | MEDLINE | ID: mdl-31089861
INTRODUCTION: In peripheral neuropathies with antibodies against Myelin Associated Glycoprotein (MAG), an IgM monoclonal gammopathy recognizes a specific epitope called Human Natural Killer 1 (HNK1) shared by NK lymphocytes and several components of the peripheral nerve myelin. Recently an ELISA test has been developed to detect antibodies against HNK1 epitope. Objectives were to determine the usefulness of this assay in the management of anti-MAG neuropathy. METHODS: Anti-HNK1 antibodies were assessed with the GanglioCombi™ MAG ELISA test (Buhlmann) in 41 anti-MAG neuropathies and in 118 controls: 34 chronic inflammatory demyelinating polyradiculoneuropathies, 3 Miller Fisher syndromes, 12 sensory neuronopathies, 63 length-dependent axonal sensory polyneuropathies, 6 healthy controls. Anti-HNK1 antibody was tested before and 1 year after rituximab therapy in seven patients with anti-MAG neuropathy. RESULTS: Anti-HNK1 antibodies were positive in 40/41 anti-MAG neuropathies, and in 1/118 controls (sensitivity 98%, specificity 99%). Only considering controls with IgM paraprotein, specificity was 96% (23/24). In anti-MAG neuropathies, anti-HNK1 titre was correlated with sensory deficiency evaluated with the INCAT sensory sum score (r = 0.4, p = 0.01) and with disability evaluated with the Rasch-built Overall Disability Scale (r = [Formula: see text] 0.4, p = 0.01) and Overall Neuropathy Limitation Scale (r = 0.4, p = 0.02). Anti-HNK1 titres were not related to age, disease duration, atypical clinical features and anti-MAG antibodies titres. Anti-MAG titres were not associated with disease severity. Anti-HNK1 titres were decreased by 18% 1 year after rituximab treatment. CONCLUSIONS: Anti-HNK1 antibodies have good sensitivity and specificity for the diagnosis of anti-MAG neuropathy. Interestingly, anti-HNK1 titres are related to the disease severity and decrease after rituximab infusions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Autoanticuerpos / Enfermedades del Sistema Nervioso Periférico / Glicoproteína Asociada a Mielina / Antígenos CD57 / Manejo de la Enfermedad Tipo de estudio: Diagnostic_studies Límite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: J Neurol Año: 2019 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Autoanticuerpos / Enfermedades del Sistema Nervioso Periférico / Glicoproteína Asociada a Mielina / Antígenos CD57 / Manejo de la Enfermedad Tipo de estudio: Diagnostic_studies Límite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: J Neurol Año: 2019 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Alemania