Pre-analytical practices for routine coagulation tests in European laboratories. A collaborative study from the European Organisation for External Quality Assurance Providers in Laboratory Medicine (EQALM).

Kristoffersen, Ann Helen; Stavelin, Anne V; Ajzner, Eva; Kristensen, Gunn B B; Sandberg, Sverre; Van Blerk, Marjan; Kitchen, Steve; Kesseler, Dagmar; Woods, Timothy A L; Meijer, Piet

Kristoffersen, Ann Helen; Stavelin, Anne V; Ajzner, Eva; Kristensen, Gunn B B; Sandberg, Sverre; Van Blerk, Marjan; Kitchen, Steve; Kesseler, Dagmar; Woods, Timothy A L; Meijer, Piet.

Afiliación

Kristoffersen AH; Department of Medical Biochemistry and Pharmacology, Haukeland University Hospital, Bergen, Norway.
Stavelin AV; Norwegian Quality Improvement of Laboratory Examinations (Noklus), Haraldsplass Deaconess Hospital, Bergen, Norway.
Ajzner E; Norwegian Quality Improvement of Laboratory Examinations (Noklus), Haraldsplass Deaconess Hospital, Bergen, Norway.
Kristensen GBB; Central Laboratory, Jósa University Hospital, Nyíregyháza, Hungary.
Sandberg S; Norwegian Quality Improvement of Laboratory Examinations (Noklus), Haraldsplass Deaconess Hospital, Bergen, Norway.
Van Blerk M; Department of Medical Biochemistry and Pharmacology, Haukeland University Hospital, Bergen, Norway.
Kitchen S; Norwegian Quality Improvement of Laboratory Examinations (Noklus), Haraldsplass Deaconess Hospital, Bergen, Norway.
Kesseler D; Department of Global Public Health and Primary Care, University of Bergen, Bergen, Norway.
Woods TAL; Quality of laboratories, Sciensano, Brussels, Belgium.
Meijer P; UK NEQAS for Blood Coagulation, Sheffield, UK.

Clin Chem Lab Med ; 57(10): 1511-1521, 2019 Sep 25.

Article en En | MEDLINE | ID: mdl-31085743

RESUMEN

Background Correct handling and storage of blood samples for coagulation tests are important to assure correct diagnosis and monitoring. The aim of this study was to assess the pre-analytical practices for routine coagulation testing in European laboratories. Methods In 2013-2014, European laboratories were invited to fill in a questionnaire addressing pre-analytical requirements regarding tube fill volume, citrate concentration, sample stability, centrifugation and storage conditions for routine coagulation testing (activated partial thromboplastin time [APTT], prothrombin time in seconds [PT-sec] and as international normalised ratio [PT-INR] and fibrinogen). Results A total of 662 laboratories from 28 different countries responded. The recommended 3.2% (105-109 mmol/L) citrate tubes are used by 74% of the laboratories. Tube fill volumes ≥90% were required by 73%-76% of the laboratories, depending upon the coagulation test and tube size. The variation in centrifugation force and duration was large (median 2500 g [10- and 90-percentiles 1500 and 4000] and 10 min [5 and 15], respectively). Large variations were also seen in the accepted storage time for different tests and sample materials, for example, for citrated blood at room temperature the accepted storage time ranged from 0.5-72 h and 0.5-189 h for PT-INR and fibrinogen, respectively. If the storage time or the tube fill requirements are not fulfilled, 72% and 84% of the respondents, respectively, would reject the samples. Conclusions There was a large variation in pre-analytical practices for routine coagulation testing in European laboratories, especially for centrifugation conditions and storage time requirements.

Asunto(s)

Pruebas de Coagulación Sanguínea/métodos; Recolección de Muestras de Sangre/métodos; Fase Preanalítica/métodos; Coagulación Sanguínea; Pruebas de Coagulación Sanguínea/normas; Recolección de Muestras de Sangre/normas; Técnicas de Laboratorio Clínico/métodos; Técnicas de Laboratorio Clínico/normas; Pruebas Diagnósticas de Rutina/métodos; Pruebas Diagnósticas de Rutina/normas; Europa (Continente); Fibrinógeno/análisis; Humanos; Laboratorios; Fase Preanalítica/normas; Tiempo de Protrombina/normas; Factores de Tiempo

Palabras clave

activated partial thromboplastin time; coagulation tests; fibrinogen; haemostasis; pre-analytical phase; prothrombin time

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas de Coagulación Sanguínea / Recolección de Muestras de Sangre / Fase Preanalítica Tipo de estudio: Diagnostic_studies Límite: Humans País/Región como asunto: Europa Idioma: En Revista: Clin Chem Lab Med Asunto de la revista: QUIMICA CLINICA / TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2019 Tipo del documento: Article País de afiliación: Noruega Pais de publicación: Alemania

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