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Cotton fiber-based assay with time-based microfluidic absorption sampling for point-of-care applications.
Wicaksono, Dedy Hb; Syazwani, Intan N; Ratnarathorn, Nalin; Sadir, Sahba; Shahir, Shafinaz; Ruckthong, Leela; Dungchai, Wijitar; Yuliarto, Brian; Dipojono, Hermawan K.
Afiliación
  • Wicaksono DH; Department of Biomedical Engineering, Faculty of Life Science & Technology, Swiss German University (SGU), The Prominence Tower, Jalan Jalur Sutera Barat no. 15, Alam Sutera, Tangerang, 15143 Banten, Indonesia.
  • Syazwani IN; Faculty of Biosciences & Medical Engineering, Universiti Teknologi Malaysia, UTM, Skudai 81310, Johor, Malaysia.
  • Ratnarathorn N; Organic Synthesis, Electrochemistry & Natural Product Research Unit, Department of Chemistry, Faculty of Science, King Mongkut's University of Technology Thonburi (KMUTT), 126 Prachautid Road, Thungkru, Bangkok 10140, Thailand.
  • Sadir S; Faculty of Biosciences & Medical Engineering, Universiti Teknologi Malaysia, UTM, Skudai 81310, Johor, Malaysia.
  • Shahir S; Faculty of Biosciences & Medical Engineering, Universiti Teknologi Malaysia, UTM, Skudai 81310, Johor, Malaysia.
  • Ruckthong L; Organic Synthesis, Electrochemistry & Natural Product Research Unit, Department of Chemistry, Faculty of Science, King Mongkut's University of Technology Thonburi (KMUTT), 126 Prachautid Road, Thungkru, Bangkok 10140, Thailand.
  • Dungchai W; Organic Synthesis, Electrochemistry & Natural Product Research Unit, Department of Chemistry, Faculty of Science, King Mongkut's University of Technology Thonburi (KMUTT), 126 Prachautid Road, Thungkru, Bangkok 10140, Thailand.
  • Yuliarto B; Department of Engineering Physics, Institut Teknologi Bandung (ITB), Bandung 40132, Indonesia.
  • Dipojono HK; Research Center for Nanosciences & Nanotechnology, Institut Teknologi Bandung (ITB), Bandung 40132, Indonesia.
Bioanalysis ; 11(9): 855-873, 2019 May.
Article en En | MEDLINE | ID: mdl-31084195
Aim: Time-based microfluidic absorption sampling was proposed using cotton fiber-based device made in swab stick. The assay was optimized and compared with conventional pipetted drop sampling using the same device. Materials & methods: Reagents were integrated into cotton fiber device for assessing concentration of analytes by the colorimetric detection method through time-based absorption sampling microfluidic system. All assay parameters were first optimized using conventional pipette-based drop sampling. Results: The color intensity is linear in the relevant concentration range of the analytes. The LOD are 0.189 mM for glucose and 6.56 µM for nitrite, respectively. These values are better than conventional drop sampling. The fiber-containing swab itself functions as sampling, assay and calibration device. Conclusion: Microfluidic cotton fiber-based assay device was fabricated and can determine analyte concentration in artificial salivary samples, colorimetrically, by time-based absorption sampling without the need of complex equipments.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sistemas de Atención de Punto / Fibra de Algodón / Dispositivos Laboratorio en un Chip / Absorción Fisicoquímica Idioma: En Revista: Bioanalysis Año: 2019 Tipo del documento: Article País de afiliación: Indonesia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sistemas de Atención de Punto / Fibra de Algodón / Dispositivos Laboratorio en un Chip / Absorción Fisicoquímica Idioma: En Revista: Bioanalysis Año: 2019 Tipo del documento: Article País de afiliación: Indonesia Pais de publicación: Reino Unido