Multiple downy mildew effectors target the stress-related NAC transcription factor LsNAC069 in lettuce.

Meisrimler, Claudia-Nicole; Pelgrom, Alexandra J E; Oud, Bart; Out, Suzan; Van den Ackerveken, Guido

Meisrimler, Claudia-Nicole; Pelgrom, Alexandra J E; Oud, Bart; Out, Suzan; Van den Ackerveken, Guido.

Afiliación

Meisrimler CN; Plant-Microbe Interactions, Department of Biology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, the Netherlands.
Pelgrom AJE; University of Canterbury, Ilam, Private Bag 4800, Christchurch, 8041, New Zealand.
Oud B; Plant-Microbe Interactions, Department of Biology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, the Netherlands.
Out S; Enza Zaden, Haling 1-E, Enkhuizen, 1602 DB, the Netherlands.
Van den Ackerveken G; Enza Zaden, Haling 1-E, Enkhuizen, 1602 DB, the Netherlands.

Plant J ; 99(6): 1098-1115, 2019 09.

Article en En | MEDLINE | ID: mdl-31077456

RESUMEN

To cause disease in lettuce, the biotrophic oomycete Bremia lactucae secretes potential RxLR effector proteins. Here we report the discovery of an effector-target hub consisting of four B. lactucae effectors and one lettuce protein target by a yeast-two-hybrid (Y2H) screening. Interaction of the lettuce tail-anchored NAC transcription factor, LsNAC069, with B. lactucae effectors does not require the N-terminal NAC domain but depends on the C-terminal region including the transmembrane domain. Furthermore, in Y2H experiments, B. lactucae effectors interact with Arabidopsis and potato tail-anchored NACs, suggesting that they are conserved effector targets. Transient expression of RxLR effector proteins BLR05 and BLR09 and their target LsNAC069 in planta revealed a predominant localization to the endoplasmic reticulum. Phytophthora capsici culture filtrate and polyethylene glycol treatment induced relocalization to the nucleus of a stabilized LsNAC069 protein, lacking the NAC-domain (LsNAC069ΔNAC ). Relocalization was significantly reduced in the presence of the Ser/Cys-protease inhibitor TPCK indicating proteolytic cleavage of LsNAC069 allows for relocalization. Co-expression of effectors with LsNAC069ΔNAC reduced its nuclear accumulation. Surprisingly, LsNAC069 silenced lettuce lines had decreased LsNAC069 transcript levels but did not show significantly altered susceptibility to B. lactucae. In contrast, LsNAC069 silencing increased resistance to Pseudomonas cichorii bacteria and reduced wilting effects under moderate drought stress, indicating a broad role of LsNAC069 in abiotic and biotic stress responses.

Asunto(s)

Lactuca/metabolismo; Oomicetos/metabolismo; Factores de Transcripción/metabolismo; Núcleo Celular/metabolismo; Resistencia a la Enfermedad; Retículo Endoplásmico/metabolismo; Regulación de la Expresión Génica/genética; Silenciador del Gen/inmunología; Interacciones Huésped-Patógeno/genética; Lactuca/genética; Oomicetos/patogenicidad; Filogenia; Enfermedades de las Plantas/microbiología; Dominios Proteicos/genética; Transporte de Proteínas/genética; Proteínas/metabolismo; Pseudomonas/patogenicidad; Estrés Fisiológico/genética; Factores de Transcripción/genética

Palabras clave

Bremia lactucae; Lactuca sativa; Pseudomonas cichorii; NAC transcription factors; drought stress; oomycetes; transcription factor relocalization

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oomicetos / Factores de Transcripción / Lactuca Idioma: En Revista: Plant J Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2019 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido

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