Your browser doesn't support javascript.
loading
Microbiota alter metabolism and mediate neurodevelopmental toxicity of 17ß-estradiol.
Catron, Tara R; Swank, Adam; Wehmas, Leah C; Phelps, Drake; Keely, Scott P; Brinkman, Nichole E; McCord, James; Singh, Randolph; Sobus, Jon; Wood, Charles E; Strynar, Mark; Wheaton, Emily; Tal, Tamara.
Afiliación
  • Catron TR; Oak Ridge Institute for Science and Education, Oak Ridge, TN, USA.
  • Swank A; U.S. EPA/ORD/NHEERL/RCU, RTP, NC, USA.
  • Wehmas LC; U.S. EPA/ORD/NHEERL/ISTD, RTP, NC, USA.
  • Phelps D; Oak Ridge Institute for Science and Education, Oak Ridge, TN, USA.
  • Keely SP; U.S. EPA/ORD/NERL/SED, Cincinnati, OH, USA.
  • Brinkman NE; U.S. EPA/ORD/NERL/SED, Cincinnati, OH, USA.
  • McCord J; Oak Ridge Institute for Science and Education, Oak Ridge, TN, USA.
  • Singh R; Oak Ridge Institute for Science and Education, Oak Ridge, TN, USA.
  • Sobus J; U.S. EPA/ORD/NERL/EMMD, RTP, NC, USA.
  • Wood CE; U.S. EPA/ORD/NHEERL/ISTD, RTP, NC, USA.
  • Strynar M; Boehringer Ingelheim Pharmaceuticals Inc., Ridgefield, CT, USA.
  • Wheaton E; U.S. EPA/ORD/NERL/EMMD, RTP, NC, USA.
  • Tal T; U.S. EPA/ORD/NERL/SED, Cincinnati, OH, USA.
Sci Rep ; 9(1): 7064, 2019 05 08.
Article en En | MEDLINE | ID: mdl-31068624
Estrogenic chemicals are widespread environmental contaminants associated with diverse health and ecological effects. During early vertebrate development, estrogen receptor signaling is critical for many different physiologic responses, including nervous system function. Recently, host-associated microbiota have been shown to influence neurodevelopment. Here, we hypothesized that microbiota may biotransform exogenous 17-ßestradiol (E2) and modify E2 effects on swimming behavior. Colonized zebrafish were continuously exposed to non-teratogenic E2 concentrations from 1 to 10 days post-fertilization (dpf). Changes in microbial composition and predicted metagenomic function were evaluated. Locomotor activity was assessed in colonized and axenic (microbe-free) zebrafish exposed to E2 using a standard light/dark behavioral assay. Zebrafish tissue was collected for chemistry analyses. While E2 exposure did not alter microbial composition or putative function, colonized E2-exposed larvae showed reduced locomotor activity in the light, in contrast to axenic E2-exposed larvae, which exhibited normal behavior. Measured E2 concentrations were significantly higher in axenic relative to colonized zebrafish. Integrated peak area for putative sulfonated and glucuronidated E2 metabolites showed a similar trend. These data demonstrate that E2 locomotor effects in the light phase are dependent on the presence of microbiota and suggest that microbiota influence chemical E2 toxicokinetics. More broadly, this work supports the concept that microbial colonization status may influence chemical toxicity.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pez Cebra / Estradiol / Microbiota / Vida Libre de Gérmenes Límite: Animals Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pez Cebra / Estradiol / Microbiota / Vida Libre de Gérmenes Límite: Animals Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido