G2-S16 sulfonate dendrimer as new therapy for treatment failure in HIV-1 entry inhibitors.
Nanomedicine (Lond)
; 14(9): 1095-1107, 2019 05.
Article
en En
| MEDLINE
| ID: mdl-31066644
Aim: Polyanionic carbosilane dendrimers have been shown to be safe and block human immunodeficiency virus type 1 (HIV-1) infection in a multifunctional manner. The aim of this study is to evaluate the appearance of HIV-1 resistance mutations after treatment with polyanionic carbosilane dendrimers. Materials & methods: A resistance mutation assay was performed on MT2 cells, viral quantity was measured by ELISA HIVp24gag and titration was carried out on TZM.bl. Next generation sequencing for HIV-1 Env was performed on G1-S4 or G2-S16 dendrimers supernatants. Results: Data showed the appearance of mutation resistance to G1-S4 treatment, inducing three significant mutations. G2-S16 did not generate any mutations and, furthermore, inhibited G1-S4-resistant viruses. Conclusion: G1-S4 treatment generates significant mutations in HIV-1NL4.3. G2-S16 does not generate resistance-associated mutation, suggesting that G2-S16 is safe as a HIV-entry inhibitor.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Silanos
/
VIH-1
/
Fármacos Anti-VIH
/
Dendrímeros
Límite:
Humans
Idioma:
En
Revista:
Nanomedicine (Lond)
Año:
2019
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Reino Unido