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TsNTxP, a non-toxic protein from Tityus serrulatus scorpion venom, induces antinociceptive effects by suppressing glutamate release in mice.
Rigo, Flávia Karine; Bochi, Guilherme Vargas; Pereira, Adriano Lana; Adamante, Gabriela; Ferro, Paula Ronsani; Dal-Toé De Prá, Samira; Milioli, Alessandra Marcone; Damiani, Adriani Paganini; da Silveira Prestes, Gabriele; Dalenogare, Diéssica Padilha; Chávez-Olórtegui, Carlos; Moraes de Andrade, Vanessa; Machado-de-Ávila, Ricardo Andrez; Trevisan, Gabriela.
Afiliación
  • Rigo FK; Graduated Program in Health Sciences, University of Southern Santa Catarina (UNESC), 88006-000, Criciúma, SC, Brazil.
  • Bochi GV; Graduated Program in Pharmacology, Federal University of Santa Maria (UFSM), 97105-900, Santa Maria, RS, Brazil.
  • Pereira AL; Graduated Program in Health Sciences, University of Southern Santa Catarina (UNESC), 88006-000, Criciúma, SC, Brazil.
  • Adamante G; Graduated Program in Health Sciences, University of Southern Santa Catarina (UNESC), 88006-000, Criciúma, SC, Brazil.
  • Ferro PR; Graduated Program in Health Sciences, University of Southern Santa Catarina (UNESC), 88006-000, Criciúma, SC, Brazil.
  • Dal-Toé De Prá S; Graduated Program in Health Sciences, University of Southern Santa Catarina (UNESC), 88006-000, Criciúma, SC, Brazil.
  • Milioli AM; Graduated Program in Health Sciences, University of Southern Santa Catarina (UNESC), 88006-000, Criciúma, SC, Brazil.
  • Damiani AP; Graduated Program in Health Sciences, University of Southern Santa Catarina (UNESC), 88006-000, Criciúma, SC, Brazil.
  • da Silveira Prestes G; Graduated Program in Health Sciences, University of Southern Santa Catarina (UNESC), 88006-000, Criciúma, SC, Brazil.
  • Dalenogare DP; Graduated Program in Pharmacology, Federal University of Santa Maria (UFSM), 97105-900, Santa Maria, RS, Brazil.
  • Chávez-Olórtegui C; Department of Biochemistry and Immunology, Federal University of Minas Gerais State (UFMG), 31270-901, Belo Horizonte, MG, Brazil.
  • Moraes de Andrade V; Graduated Program in Health Sciences, University of Southern Santa Catarina (UNESC), 88006-000, Criciúma, SC, Brazil.
  • Machado-de-Ávila RA; Graduated Program in Health Sciences, University of Southern Santa Catarina (UNESC), 88006-000, Criciúma, SC, Brazil.
  • Trevisan G; Graduated Program in Health Sciences, University of Southern Santa Catarina (UNESC), 88006-000, Criciúma, SC, Brazil; Graduated Program in Pharmacology, Federal University of Santa Maria (UFSM), 97105-900, Santa Maria, RS, Brazil. Electronic address: gabriela.trevisan-santos@ufsm.br.
Eur J Pharmacol ; 855: 65-74, 2019 Jul 15.
Article en En | MEDLINE | ID: mdl-31059709
Neuropathic pain is a common type of chronic pain caused by trauma or chemotherapy. However, this type of pain is undertreated. TsNTxP is a non-toxic protein isolated from the venom of the scorpion Tityus serrulatus, and it is structurally similar to neurotoxins that interact with voltage-gated sodium channels. However, the antinociceptive properties of this protein have not been characterized. The purpose of this study was to investigate the antinociceptive effects of TsNTxP in acute and neuropathic pain models. Male and female Swiss mice (25-30 g) were exposed to different models of acute pain (tail-flick test and nociception caused by capsaicin intraplantar injection) or neuropathic pain (chronic pain syndrome induced by paclitaxel or chronic constriction injury of the sciatic nerve). Hypersensitivity to mechanical or cold stimuli were evaluated in the models of neuropathic pain. The ability of TsNTxP to alter the release of glutamate in mouse spinal cord synaptosomes was also evaluated. The results showed that TsNTxP exerted antinociceptive effects in the tail-flick test to a thermal stimulus and in the intraplantar capsaicin administration model. Furthermore, TsNTxP was non-toxic and exerted antiallodynic effects in neuropathic pain models induced by chronic constriction injury of the sciatic nerve and administration of paclitaxel. TsNTxP reduced glutamate release from mouse spinal cord synaptosomes following stimulation with potassium chloride (KCl) or capsaicin. Thus, this T. serrulatus protein may be a promising non-toxic drug for the treatment of neuropathic pain.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Venenos de Escorpión / Escorpiones / Ácido Glutámico / Proteínas de Artrópodos / Analgésicos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Eur J Pharmacol Año: 2019 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Venenos de Escorpión / Escorpiones / Ácido Glutámico / Proteínas de Artrópodos / Analgésicos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Eur J Pharmacol Año: 2019 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos