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Attenuation of Endoplasmic Reticulum Stress, Impaired Calcium Homeostasis, and Altered Bioenergetic Functions in MPP+-Exposed SH-SY5Y Cells Pretreated with Rutin.
Enogieru, Adaze Bijou; Haylett, William Lloyd; Miller, Hayley Christy; van der Westhuizen, Francois Hendrikus; Hiss, Donavon Charles; Ekpo, Okobi Eko.
Afiliación
  • Enogieru AB; Department of Medical Biosciences, University of the Western Cape, Robert Sobukwe Road, Private Bag X17, Bellville, 7535, South Africa.
  • Haylett WL; Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.
  • Miller HC; Division of Endocrinology, Department of Medicine, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.
  • van der Westhuizen FH; Focus Area for Human Metabolomics, Faculty of Natural Sciences, North-West University, Potchefstroom, South Africa.
  • Hiss DC; Focus Area for Human Metabolomics, Faculty of Natural Sciences, North-West University, Potchefstroom, South Africa.
  • Ekpo OE; Department of Medical Biosciences, University of the Western Cape, Robert Sobukwe Road, Private Bag X17, Bellville, 7535, South Africa.
Neurotox Res ; 36(4): 764-776, 2019 Nov.
Article en En | MEDLINE | ID: mdl-31055769
Parkinson's disease (PD) is a common neurodegenerative disorder that affects approximately 1% of the population over the age of 65 years. While treatment options for PD are limited, reports show that plant-derived bioactive compounds such as rutin possess numerous pharmacological benefits, including antioxidant and antiapoptotic activities. This study aimed to investigate the potential role of rutin in MPP+-treated SH-SY5Y neuroblastoma cells, an established cell model of PD. Our findings reveal increased concentrations of Ca2+ and endoplasmic reticulum (ER) stress as well as impaired mitochondrial membrane potential and bioenergetic status in SH-SY5Y cells treated with MPP+ only. This is demonstrated by a significant reduction in the expression levels of BiP, significantly reduced basal respiration, maximal respiration, and spare respiratory capacity as well as a significant increase in the expression levels of CHOP; however, these effects were significantly attenuated following pretreatment with rutin. Also, rutin significantly improved basal and compensatory glycolysis as a response to an impaired oxidative phosphorylation system triggered by MPP+, characterized by deficient ATP production. In conclusion, our findings provide the first evidence on the ability of rutin to maintain Ca2+ homeostasis, inhibit ER stress, and protect the mitochondria in MPP+-treated SH-SY5Y cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedad de Parkinson Secundaria / Rutina / 1-Metil-4-fenilpiridinio / Calcio / Estrés del Retículo Endoplásmico / Mitocondrias / Antioxidantes Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Neurotox Res Asunto de la revista: NEUROLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Sudáfrica Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedad de Parkinson Secundaria / Rutina / 1-Metil-4-fenilpiridinio / Calcio / Estrés del Retículo Endoplásmico / Mitocondrias / Antioxidantes Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Neurotox Res Asunto de la revista: NEUROLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Sudáfrica Pais de publicación: Estados Unidos