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Enemas with mesalazine increase the tissue contents of mucins in the colonic mucosa devoid of fecal stream.
Martinez, Carlos Augusto Real; Campos, Fábio Guilherme; Kanno, Danilo Toshio; Meneses, Eli Cristiano; Matijascic, Gabrielle Maira; Goto, Eduardo Felipe Kim; Pereira, José Aires.
Afiliación
  • Martinez CAR; PhD, Associate Professor, Postgraduate Program in Health Sciences, Universidade São Francisco (USF), Bragança Paulista-SP, and Department of Surgery, Universidade Estadual de Campinas (UNICAMP), Campinas-SP, Brazil. Conception and design of the study, statistics analysis, interpretation of data, man
  • Campos FG; PhD, Associate Professor, Department of Gastroenterology, Faculty of Medicine, Universidade de São Paulo (USP), Brazil. Interpretation of data, critical revision.
  • Kanno DT; Fellow Master degree, Assistant Professor, Division of Surgery, Faculty of Medicine, USF, Bragança Paulista-SP, Brazil. Technical procedures, acquisition of data.
  • Meneses EC; Fellow Master degree, Assistant Professor, Faculty of Pharmacology, USF, Bragança Paulista-SP, Brazil. Technical procedures, acquisition of data.
  • Matijascic GM; Graduate student, Faculty of Medicine, USF, Bragança Paulista-SP, Brazil. Technical procedures, acquisition of data.
  • Goto EFK; Graduate student, Faculty of Medicine, USF, Bragança Paulista-SP, Brazil. Technical procedures, acquisition of data.
  • Pereira JA; PhD, Assistant Professor, Division of Pathology, Faculty of Medicine, USF, Bragança Paulista-SP, Brazil. Histopathological examinations, acquisition and interpretation of data.
Acta Cir Bras ; 34(4): e201900406, 2019 Apr 29.
Article en En | MEDLINE | ID: mdl-31038584
PURPOSE: To evaluate the inflammatory reaction and measure the content of mucins, in the colonic mucosa without fecal stream submit to intervention with mesalazine. METHODS: Twenty-four rats were submitted to a left colostomy and a distal mucous fistula and divided into two groups according to euthanasia to be performed two or four weeks. Each group was divided into two subgroups according daily application of enemas containing saline or mesalazine at 1.0 g/kg/day. Colitis was diagnosed by histological analysis and the inflammatory reaction by validated score. Acidic mucins and neutral mucins were determined with the alcian-blue and periodic acid of Schiff techniques, respectively. Sulfomucin and sialomucin were identified by high iron diamine-alcian blue technique. The tissue contents of mucins were quantified by computer-assisted image analysis. Mann-Whitney test was used to analyze the results establishing the level of significance of 5%. RESULTS: Enemas with mesalazine in colonic segments without fecal stream decreased the inflammation score and increased the tissue content of all subtypes of mucins. The increase of tissue content of neutral, acid and sulfomucin was related to the time of intervention. CONCLUSION: Mesalazine enemas reduce the inflammatory process and preserve the content of mucins in colonic mucosa devoid of fecal stream.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Antiinflamatorios no Esteroideos / Colon / Mesalamina / Enema / Mucosa Intestinal / Mucinas Tipo de estudio: Evaluation_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Acta Cir Bras Año: 2019 Tipo del documento: Article Pais de publicación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Antiinflamatorios no Esteroideos / Colon / Mesalamina / Enema / Mucosa Intestinal / Mucinas Tipo de estudio: Evaluation_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Acta Cir Bras Año: 2019 Tipo del documento: Article Pais de publicación: Brasil