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Preparation of surface protein imprinted thermosensitive polymer monolithic column and its specific adsorption for BSA.
Wang, Jiqi; Huyan, Yu; Yang, Zuoting; Zhang, Hepeng; Zhang, Aibo; Kou, Xiaokang; Zhang, Qiuyu; Zhang, Baoliang.
Afiliación
  • Wang J; School of Applied and Natural Sciences, Northwestern Polytechnical University, Xi'an 710129, PR China.
  • Huyan Y; School of Applied and Natural Sciences, Northwestern Polytechnical University, Xi'an 710129, PR China.
  • Yang Z; School of Applied and Natural Sciences, Northwestern Polytechnical University, Xi'an 710129, PR China.
  • Zhang H; School of Applied and Natural Sciences, Northwestern Polytechnical University, Xi'an 710129, PR China; MOE Key Laboratory of Material Physics and Chemistry Under Extraordinary Condition, Ministry of Education, Northwestern Polytechnical University, Xi'an 710072, PR China.
  • Zhang A; School of Applied and Natural Sciences, Northwestern Polytechnical University, Xi'an 710129, PR China; MOE Key Laboratory of Material Physics and Chemistry Under Extraordinary Condition, Ministry of Education, Northwestern Polytechnical University, Xi'an 710072, PR China.
  • Kou X; Sunresin New Materiasls Co. Ltd., Xi'an 710072, PR China.
  • Zhang Q; School of Applied and Natural Sciences, Northwestern Polytechnical University, Xi'an 710129, PR China; MOE Key Laboratory of Material Physics and Chemistry Under Extraordinary Condition, Ministry of Education, Northwestern Polytechnical University, Xi'an 710072, PR China.
  • Zhang B; School of Applied and Natural Sciences, Northwestern Polytechnical University, Xi'an 710129, PR China; Sunresin New Materiasls Co. Ltd., Xi'an 710072, PR China. Electronic address: blzhang@nwpu.edu.cn.
Talanta ; 200: 526-536, 2019 Aug 01.
Article en En | MEDLINE | ID: mdl-31036219
In this work, a novel thermosensitive surface protein imprinted polymer monolithic column (TsIPMC) was synthesized by combining high internal phase emulsion with 1,1-diphenylethene (DPE) controlled polymerization. Innovatively, DPE and acrylic acid (AA) monomers were introduced in high internal oil and water phases respectively. The research showed that DPE could not only initiate the polymerization of monomers, but also improve the pore performance of monolithic columns. The elution efficiency of template or target protein could be significantly improved by the thermoresponse characteristics of TsIPMC. The effects of DPE and AA on adsorption capacity and imprinting factor (IF) were studied. The optimization results presented that the optimal addition amounts were 55 mg and 50 mg. Under such conditions, the IF of as-prepared TsIPMC was 1.61 and the saturated adsorption capacity was 66 mg/mL. The influences of the flow rate and target protein concentration on the adsorption equilibrium time and effluent volume were revealed. TsIPMC showed higher selectivity for different competing proteins. The reuse stability result showed that the adsorption of TsIPMC to BSA decreased by 3.69% after 12 times of reuse, and the IF remained basically unchanged. TsIPMC would demonstrate the potential applications in the field of protein purification and separation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Temperatura / Albúmina Sérica Bovina / Impresión Molecular / Proteínas de la Membrana Límite: Animals Idioma: En Revista: Talanta Año: 2019 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Temperatura / Albúmina Sérica Bovina / Impresión Molecular / Proteínas de la Membrana Límite: Animals Idioma: En Revista: Talanta Año: 2019 Tipo del documento: Article Pais de publicación: Países Bajos