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GRP94 promotes muscle differentiation by inhibiting the PI3K/AKT/mTOR signaling pathway.
Li, Shuang; Fu, Yuying; Pang, Yusheng; Tong, Huili; Li, Shufeng; Yan, Yunqin.
Afiliación
  • Li S; The Laboratory of Cell and Development, Northeast Agricultural University, Harbin, Heilongjiang, China.
  • Fu Y; The Laboratory of Cell and Development, Northeast Agricultural University, Harbin, Heilongjiang, China.
  • Pang Y; The Laboratory of Cell and Development, Northeast Agricultural University, Harbin, Heilongjiang, China.
  • Tong H; The Laboratory of Cell and Development, Northeast Agricultural University, Harbin, Heilongjiang, China.
  • Li S; The Laboratory of Cell and Development, Northeast Agricultural University, Harbin, Heilongjiang, China.
  • Yan Y; The Laboratory of Cell and Development, Northeast Agricultural University, Harbin, Heilongjiang, China.
J Cell Physiol ; 234(11): 21211-21223, 2019 11.
Article en En | MEDLINE | ID: mdl-31025379
The glucose-regulated endoplasmic reticulum chaperone protein 94 (GRP94) is required for many biological processes, such as secretion of immune factors and mesoderm induction. Here, we demonstrated that GRP94 promotes muscle differentiation in vitro and in vivo. Moreover, GRP94 inhibited the PI3K/AKT/mTOR signaling pathway. Using both in vitro and in vivo approaches, in myoblasts, we found that this inhibition resulted in reduced proliferation and increased differentiation. To further investigate the mechanism of GRP94-induced muscle differentiation, we used co-immunoprecipitation and proximity ligation assays and found that GRP94 interacted with PI3K-interacting protein 1 (Pik3ip1). The latter protein promoted muscle differentiation by inhibiting the PI3K/AKT/mTOR pathway. Furthermore, GRP94 was found to regulate Pik3ip1 expression. Finally, when Pik3ip1 expression was inhibited, GRP94-induced promotion of muscle differentiation was diminished. Taken together, our data demonstrated that GRP94 promoted muscle differentiation, mediated by Pik3ip1-dependent inhibition of the PI3K/AKT/mTOR signaling pathway.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicoproteínas de Membrana / Transducción de Señal / Células Musculares / Mioblastos Límite: Animals Idioma: En Revista: J Cell Physiol Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicoproteínas de Membrana / Transducción de Señal / Células Musculares / Mioblastos Límite: Animals Idioma: En Revista: J Cell Physiol Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos