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Salting out assisted liquid-liquid extraction for liquid chromatography tandem-mass spectrometry determination of amphetamine-like stimulants in meconium.
Nemeskalová, Alzbeta; Bursová, Miroslava; Sýkora, David; Kuchar, Martin; Cabala, Radomír; Hlozek, Tomás.
Afiliación
  • Nemeskalová A; Department of Analytical Chemistry, University of Chemistry and Technology in Prague, Technická 5, 166 28 Prague 6, Czech Republic; Forensic Laboratory of Biologically Active Substances, Department of Chemistry of Natural Compounds, University of Chemistry and Technology in Prague, Czech Republic; N
  • Bursová M; Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 2, 121 08 Prague 2, Czech Republic.
  • Sýkora D; Department of Analytical Chemistry, University of Chemistry and Technology in Prague, Technická 5, 166 28 Prague 6, Czech Republic.
  • Kuchar M; Forensic Laboratory of Biologically Active Substances, Department of Chemistry of Natural Compounds, University of Chemistry and Technology in Prague, Czech Republic; National Institute of Mental Health, Topolová 748, 250 67 Klecany, Czech Republic. Electronic address: Martin.Kuchar@vscht.cz.
  • Cabala R; Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 2, 121 08 Prague 2, Czech Republic; Department of Analytical Chemistry, Faculty of Science, Charles University, Albertov 6, 128 43 Prague 2, Czech Republ
  • Hlozek T; Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 2, 121 08 Prague 2, Czech Republic; Department of Analytical Chemistry, Faculty of Science, Charles University, Albertov 6, 128 43 Prague 2, Czech Republ
J Pharm Biomed Anal ; 172: 42-49, 2019 Aug 05.
Article en En | MEDLINE | ID: mdl-31022615
In the last decade there has been a dramatic increase in the availability and abuse of synthetic cathinones - new amphetamine-like stimulants. Even though their abuse during pregnancy could have serious adverse effects on the fetus, cathinones are not readily included in neonatal toxicological screenings. Meconium (first neonatal stool) is the specimen of choice to reveal long term drug exposure, however as it is a highly complex matrix, the sample preparation is a critical step before the instrumental analysis. The aim of this work was to develop a suitable meconium sample extraction technique using the advantages of salting-out assisted liquid-liquid extraction (SALLE) and using only MS-friendly organic ammonium salts. We further developed and validated liquid chromatography tandem-mass spectrometry method for the determination of 'traditional' stimulants (methamphetamine, amphetamine, MDMA) and cathinones (mephedrone, methylenedioxypyrovalerone (MDPV), α-pyrrolidinopentiophenone (α-PVP), methylone, butylone, flephedrone, and naphyrone). Matrix-matched calibration was prepared in the concentration range 10-2000 ng/g. The limits of quantification were determined as 10 ng/g, recoveries ranged from 48.2% to 94.3% and the matrix effect was between 60.2% and 101.4%. Accuracy (86.1-114.5%) and precision (4.9-14.9%) were determined and all validation criteria were met for all analytes except for naphyrone. Finally, our analytical method was tested on a set of real meconium samples, which were found positive for amphetamine, methamphetamine and methylone, thus demonstrating the validity of the method.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Detección de Abuso de Sustancias / Extracción Líquido-Líquido / Anfetaminas / Estimulantes del Sistema Nervioso Central / Meconio Tipo de estudio: Diagnostic_studies Límite: Female / Humans / Newborn / Pregnancy Idioma: En Revista: J Pharm Biomed Anal Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Detección de Abuso de Sustancias / Extracción Líquido-Líquido / Anfetaminas / Estimulantes del Sistema Nervioso Central / Meconio Tipo de estudio: Diagnostic_studies Límite: Female / Humans / Newborn / Pregnancy Idioma: En Revista: J Pharm Biomed Anal Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido