Polarized Two-Photon Absorption and Heterogeneous Fluorescence Dynamics in NAD(P)H.

Blacker, Thomas S; Nicolaou, Nick; Duchen, Michael R; Bain, Angus J

Blacker, Thomas S; Nicolaou, Nick; Duchen, Michael R; Bain, Angus J.

Afiliación

Blacker TS; Department of Physics & Astronomy , University College London , Gower Street , London WC1E 6BT , United Kingdom.
Nicolaou N; Centre for Mathematics and Physics in the Life Sciences and Experimental Biology (CoMPLEX) , University College London , Gower Street , London WC1E 6BT , United Kingdom.
Duchen MR; Research Department of Cell & Developmental Biology , University College London , Gower Street , London WC1E 6BT , United Kingdom.
Bain AJ; Department of Physics & Astronomy , University College London , Gower Street , London WC1E 6BT , United Kingdom.

J Phys Chem B ; 123(22): 4705-4717, 2019 06 06.

Article en En | MEDLINE | ID: mdl-31021092

RESUMEN

Two-photon absorption (2PA) finds widespread application in biological systems, which frequently exhibit heterogeneous fluorescence decay dynamics corresponding to multiple species or environments. By combining polarized 2PA with time-resolved fluorescence intensity and anisotropy decay measurements, we show how the two-photon transition tensors for the components of a heterogeneous population can be separately determined, allowing structural differences between the two fluorescent states of the redox cofactor NAD(P)H to be identified. The results support the view that the two states correspond to alternate configurations of the nicotinamide ring, rather than folded and extended conformations of the entire molecule.

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Phys Chem B Asunto de la revista: QUIMICA Año: 2019 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google