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Parabolic, Flight-Induced, Acute Hypergravity and Microgravity Effects on the Beating Rate of Human Cardiomyocytes.
Acharya, Aviseka; Brungs, Sonja; Lichterfeld, Yannick; Hescheler, Jürgen; Hemmersbach, Ruth; Boeuf, Helene; Sachinidis, Agapios.
Afiliación
  • Acharya A; Institute of Neurophysiology, Center for Molecular Medicine Cologne (CMMC), University of Cologne, 50931 Cologne, Germany. aacharya@uni-koeln.de.
  • Brungs S; German Aerospace Center, Institute of Aerospace Medicine, Gravitational Biology, Linder Hoehe, 51147 Cologne, Germany. Sonja.Brungs@dlr.de.
  • Lichterfeld Y; German Aerospace Center, Institute of Aerospace Medicine, Gravitational Biology, Linder Hoehe, 51147 Cologne, Germany. Yannick.Lichterfeld@dlr.de.
  • Hescheler J; Institute of Neurophysiology, Center for Molecular Medicine Cologne (CMMC), University of Cologne, 50931 Cologne, Germany. j.hescheler@uni-koeln.de.
  • Hemmersbach R; German Aerospace Center, Institute of Aerospace Medicine, Gravitational Biology, Linder Hoehe, 51147 Cologne, Germany. Ruth.Hemmersbach@dlr.de.
  • Boeuf H; INSERM (French National Institute of Health and Medical Research), U1026-Biotis, Université de Bordeaux, 33076 Bordeaux, France. helene.boeuf@u-bordeaux.fr.
  • Sachinidis A; Institute of Neurophysiology, Center for Molecular Medicine Cologne (CMMC), University of Cologne, 50931 Cologne, Germany. a.sachinidis@uni-koeln.de.
Cells ; 8(4)2019 04 14.
Article en En | MEDLINE | ID: mdl-31013958
Functional studies of human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (hCMs) under different gravity conditions contribute to aerospace medical research. To study the effects of altered gravity on hCMs, we exposed them to acute hypergravity and microgravity phases in the presence and absence of the ß-adrenoceptor isoprenalin (ISO), L-type Ca2+ channel (LTCC) agonist Bay-K8644, or LTCC blocker nifedipine, and monitored their beating rate (BR). These logistically demanding experiments were executed during the 66th Parabolic Flight Campaign of the European Space Agency. The hCM cultures were exposed to 31 alternating hypergravity, microgravity, and hypergravity phases, each lasting 20-22 s. During the parabolic flight experiment, BR and cell viability were monitored using the xCELLigence real-time cell analyzer Cardio Instrument®. Corresponding experiments were performed on the ground (1 g), using an identical set-up. Our results showed that BR continuously increased during the parabolic flight, reaching a 40% maximal increase after 15 parabolas, compared with the pre-parabolic (1 g) phase. However, in the presence of the LTCC blocker nifedipine, no change in BR was observed, even after 31 parabolas. We surmise that the parabola-mediated increase in BR was induced by the LTCC blocker. Moreover, the increase in BR induced by ISO and Bay-K8644 during the pre-parabola phase was further elevated by 20% after 25 parabolas. This additional effect reflects the positive impact of the parabolas in the absence of both agonists. Our study suggests that acute alterations of gravity significantly increase the BR of hCMs via the LTCC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ingravidez / Hipergravedad / Miocitos Cardíacos Límite: Humans Idioma: En Revista: Cells Año: 2019 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ingravidez / Hipergravedad / Miocitos Cardíacos Límite: Humans Idioma: En Revista: Cells Año: 2019 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza