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[miR-494-3p reduces insulin sensitivity in diabetic cardiomyocytes by down-regulation of insulin receptor substrate 1].
Wu, Jie; Qin, Xing-Hua; Hou, Zuo-Xu; Fu, Zi-Hao; Li, Guo-Hua; Yang, Hong-Yan; Zhang, Xing; Gao, Feng.
Afiliación
  • Wu J; School of Aerospace Medicine, the Fourth Military Medical University, Xi'an 710032, China.
  • Qin XH; School of Aerospace Medicine, the Fourth Military Medical University, Xi'an 710032, China.
  • Hou ZX; School of Aerospace Medicine, the Fourth Military Medical University, Xi'an 710032, China.
  • Fu ZH; School of Aerospace Medicine, the Fourth Military Medical University, Xi'an 710032, China.
  • Li GH; School of Aerospace Medicine, the Fourth Military Medical University, Xi'an 710032, China.
  • Yang HY; School of Aerospace Medicine, the Fourth Military Medical University, Xi'an 710032, China.
  • Zhang X; School of Aerospace Medicine, the Fourth Military Medical University, Xi'an 710032, China. zhangxing@fmmu.edu.cn.
  • Gao F; School of Aerospace Medicine, the Fourth Military Medical University, Xi'an 710032, China.
Sheng Li Xue Bao ; 71(2): 271-278, 2019 Apr 25.
Article en Zh | MEDLINE | ID: mdl-31008486
More and more evidence suggests that microRNA is widely involved in the regulation of cardiovascular function. Our preliminary experiment showed that miR-494-3p was increased in heart of diabetic rats, and miR-494-3p was reported to be related to metabolism such as obesity and exercise. Therefore, this study was aimed to explore the role of miR-494-3p in diabetic myocardial insulin sensitivity and the related mechanism. The diabetic rat model was induced by high fat diet (45 kcal% fat, 12 weeks) combined with streptozotocin (STZ, 30 mg/kg), and cardiac tissue RNA was extracted for qPCR. The results showed that the level of miR-494-3p was significantly up-regulated in the myocardium of diabetic rats compared with the control (P < 0.05). The level of miR-494-3p in H9c2 cells cultured in high glucose and high fat medium (HGHF) was significantly increased (P < 0.01) with the increase of sodium palmitate concentration, whereas down-regulation of miR-494-3p in HGHF treated cells led to an increase in insulin-stimulated glucose uptake (P < 0.01) and the ratio of p-Akt/Akt (P < 0.05). Over-expression of miR-494-3p in H9c2 cell line significantly inhibited insulin-stimulated glucose uptake and phosphorylation of Akt (P < 0.01). Bioinformatics combined with Western blotting experiments confirmed insulin receptor substrate 1 (IRS1) as a target molecule of miR-494-3p. These results suggest that miR-494-3p reduces insulin sensitivity in diabetic cardiomyocytes by down-regulating IRS1.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Resistencia a la Insulina / Miocitos Cardíacos / MicroARNs / Diabetes Mellitus Experimental / Proteínas Sustrato del Receptor de Insulina Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: Zh Revista: Sheng Li Xue Bao Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: China
Buscar en Google
Añadir a Mi BVS
Imprimir
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PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Resistencia a la Insulina / Miocitos Cardíacos / MicroARNs / Diabetes Mellitus Experimental / Proteínas Sustrato del Receptor de Insulina Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: Zh Revista: Sheng Li Xue Bao Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: China