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Increased luminescence of the GloSensor cAMP assay in LßT2 cells does not correlate with cAMP accumulation under low pH conditions.
Musha, Shiori; Murakami, Syo; Kojima, Ryotaro; Tomura, Hideaki.
Afiliación
  • Musha S; Laboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki 214-8571, Japan.
  • Murakami S; Laboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki 214-8571, Japan.
  • Kojima R; Laboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki 214-8571, Japan.
  • Tomura H; Laboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki 214-8571, Japan.
J Reprod Dev ; 65(4): 381-388, 2019 Aug 09.
Article en En | MEDLINE | ID: mdl-31006726
Cyclic adenosine monophosphate (cAMP) plays a pivotal role in gonadotrope responses in the pituitary. Gonadotropin-releasing hormone (GnRH) mediated synthesis and secretion of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) are regulated by both the Gs/cAMP and Gq/Ca2+ signaling pathways. Pituitary adenylate cyclase-activating polypeptide (PACAP) also regulates GnRH responsiveness in gonadotropes through the PACAP receptor, which activates the Gs/cAMP signaling pathway. Therefore, measuring intracellular cAMP levels is important for elucidating the molecular mechanisms of FSH and LH synthesis and secretion in gonadotropes. The GloSensor cAMP assay is useful for detecting cAMP levels in intact, living cells. In this study, we found that increased GloSensor luminescence intensity did not correlate with cAMP accumulation in LßT2 cells under low pH conditions. This result indicates that cell type and condition must be considered when using GloSensor cAMP.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bioensayo / AMP Cíclico / Gonadotrofos / Mediciones Luminiscentes Tipo de estudio: Evaluation_studies Límite: Animals / Humans Idioma: En Revista: J Reprod Dev Asunto de la revista: MEDICINA REPRODUTIVA Año: 2019 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bioensayo / AMP Cíclico / Gonadotrofos / Mediciones Luminiscentes Tipo de estudio: Evaluation_studies Límite: Animals / Humans Idioma: En Revista: J Reprod Dev Asunto de la revista: MEDICINA REPRODUTIVA Año: 2019 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón