RNAi expression tuning, microfluidic screening, and genome recombineering for improved protein production in <i>Saccharomyces cerevisiae</i>.

Wang, Guokun; Björk, Sara M; Huang, Mingtao; Liu, Quanli; Campbell, Kate; Nielsen, Jens; Joensson, Haakan N; Petranovic, Dina

RNAi expression tuning, microfluidic screening, and genome recombineering for improved protein production in Saccharomyces cerevisiae.

Wang, Guokun; Björk, Sara M; Huang, Mingtao; Liu, Quanli; Campbell, Kate; Nielsen, Jens; Joensson, Haakan N; Petranovic, Dina.

Afiliación

Wang G; Department of Biology and Biological Engineering, Chalmers University of Technology, SE41296 Gothenburg, Sweden.
Björk SM; Science for Life Laboratory, Division of Protein Science, School of Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, SE17165 Solna, Sweden.
Huang M; Novo Nordisk Foundation Center for Biosustainability, KTH Royal Institute of Technology, SE17165 Solna, Sweden.
Liu Q; Department of Biology and Biological Engineering, Chalmers University of Technology, SE41296 Gothenburg, Sweden.
Campbell K; Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, SE41296 Gothenburg, Sweden.
Nielsen J; Department of Biology and Biological Engineering, Chalmers University of Technology, SE41296 Gothenburg, Sweden.
Joensson HN; Department of Biology and Biological Engineering, Chalmers University of Technology, SE41296 Gothenburg, Sweden.
Petranovic D; Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, SE41296 Gothenburg, Sweden.

Proc Natl Acad Sci U S A ; 116(19): 9324-9332, 2019 05 07.

Article en En | MEDLINE | ID: mdl-31000602

Asunto(s)

Interferencia de ARN; Proteínas Recombinantes/biosíntesis; Saccharomyces cerevisiae/genética; Saccharomyces cerevisiae/metabolismo; Genoma Fúngico; Microfluídica; Proteínas Recombinantes/genética; Recombinación Genética; Saccharomyces cerevisiae/química; Proteínas de Saccharomyces cerevisiae/genética; Proteínas de Saccharomyces cerevisiae/metabolismo

Palabras clave

RNA interference; Saccharomyces cerevisiae; droplet microfluidic screening; genome recombineering; protein production

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Proteínas Recombinantes / Interferencia de ARN Tipo de estudio: Diagnostic_studies / Prognostic_studies / Screening_studies Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2019 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos

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