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Detection of dicistroviruses RNA in blood of febrile Tanzanian children.
Cordey, Samuel; Laubscher, Florian; Hartley, Mary-Anne; Junier, Thomas; Pérez-Rodriguez, Francisco J; Keitel, Kristina; Vieille, Gael; Samaka, Josephine; Mlaganile, Tarsis; Kagoro, Frank; Boillat-Blanco, Noémie; Mbarack, Zainab; Docquier, Mylène; Brito, Francisco; Eibach, Daniel; May, Jürgen; Sothmann, Peter; Aldrich, Cassandra; Lusingu, John; Tapparel, Caroline; D'Acremont, Valérie; Kaiser, Laurent.
Afiliación
  • Cordey S; a Division of Infectious Diseases and Laboratory of Virology , University of Geneva Hospitals Geneva , Switzerland.
  • Laubscher F; b University of Geneva Medical School Geneva , Switzerland.
  • Hartley MA; a Division of Infectious Diseases and Laboratory of Virology , University of Geneva Hospitals Geneva , Switzerland.
  • Junier T; b University of Geneva Medical School Geneva , Switzerland.
  • Pérez-Rodriguez FJ; c Department of Ambulatory Care and Community Medicine , Lausanne University Hospital Lausanne , Switzerland.
  • Keitel K; d Swiss Institute of Bioinformatics Geneva , Switzerland.
  • Vieille G; e Global Health Institute, School of Life Sciences , École Polytechnique Fédérale de Lausanne Lausanne , Switzerland.
  • Samaka J; b University of Geneva Medical School Geneva , Switzerland.
  • Mlaganile T; f Swiss Tropical and Public Health Institute , University of Basel Basel , Switzerland.
  • Kagoro F; a Division of Infectious Diseases and Laboratory of Virology , University of Geneva Hospitals Geneva , Switzerland.
  • Boillat-Blanco N; b University of Geneva Medical School Geneva , Switzerland.
  • Mbarack Z; g Ifakara Health Institute , Dar es Salaam , Tanzania.
  • Docquier M; h Amana Hospital , Dar es Salaam , Tanzania.
  • Brito F; g Ifakara Health Institute , Dar es Salaam , Tanzania.
  • Eibach D; g Ifakara Health Institute , Dar es Salaam , Tanzania.
  • May J; f Swiss Tropical and Public Health Institute , University of Basel Basel , Switzerland.
  • Sothmann P; i Infectious Diseases Service , Lausanne University Hospital Lausanne , Switzerland.
  • Aldrich C; j Mwananyamala Hospital , Dar es Salaam , Tanzania.
  • Lusingu J; k iGE3 Genomics Platform , University of Geneva Geneva , Switzerland.
  • Tapparel C; d Swiss Institute of Bioinformatics Geneva , Switzerland.
  • D'Acremont V; l Department of Genetic Medicine and Development , Faculty of Medicine of Geneva Geneva , Switzerland.
  • Kaiser L; m Department of Infectious Disease Epidemiology , Bernhard Nocht Institute for Tropical Medicine Hamburg , Germany.
Emerg Microbes Infect ; 8(1): 613-623, 2019.
Article en En | MEDLINE | ID: mdl-30999808
Fever is the leading cause of paediatric outpatient consultations in Sub-Saharan Africa. Although most are suspected to be of viral origin, a putative causative pathogen is not identified in over a quarter of these febrile episodes. Using a de novo assembly sequencing approach, we report the detection (15.4%) of dicistroviruses (DicV) RNA in sera collected from 692 febrile Tanzanian children. In contrast, DicV RNA was only detected in 1/77 (1.3%) plasma samples from febrile Tanzanian adults, suggesting that children could represent the primary susceptible population. Estimated viral load by specific quantitative real-time RT-PCR assay ranged from < 1.32E3 to 1.44E7 viral RNA copies/mL serum. Three DicV full-length genomes were obtained, and a phylogenetic analyse on the capsid region showed the presence of two clusters representing tentative novel genus. Although DicV-positive cases were detected throughout the year, a significantly higher positivity rate was observed during the rainy season. This study reveals that novel DicV RNA is frequently detected in the blood of Tanzanian children, paving the way for further investigations to determine if DicV possibly represent a new agent in humans.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus / Virosis / ARN Viral / Fiebre Tipo de estudio: Diagnostic_studies / Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Child / Child, preschool / Female / Humans / Infant / Male País/Región como asunto: Africa Idioma: En Revista: Emerg Microbes Infect Año: 2019 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus / Virosis / ARN Viral / Fiebre Tipo de estudio: Diagnostic_studies / Etiology_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Child / Child, preschool / Female / Humans / Infant / Male País/Región como asunto: Africa Idioma: En Revista: Emerg Microbes Infect Año: 2019 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Estados Unidos