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[Determination of thresholds values for platelet serotonin and urinary 5-HIAA concentrations for the biological diagnosis of digestive neuroendocrine tumors]. / Quelles valeurs de référence pour la concentration de la sérotonine plaquettaire et du 5-HIAA urinaire pour le diagnostic des tumeurs neuroendocrines digestives ?
Padelli, Maël; Bruno, Clément; Lemarchand, Julia; Vourc'h, Patrick; Andres, Christian; Blasco, Hélène; Benz-de Bretagne, Isabelle.
Afiliación
  • Padelli M; Laboratoire de biochimie et biologie moléculaire, CHRU de Tours, France.
  • Bruno C; Laboratoire de biochimie et biologie moléculaire, CHRU de Tours, France, UMR 1253, iBrain, Université de Tours, Inserm, Tours, France.
  • Lemarchand J; Laboratoire de biochimie et biologie moléculaire, CHRU de Tours, France.
  • Vourc'h P; Laboratoire de biochimie et biologie moléculaire, CHRU de Tours, France, UMR 1253, iBrain, Université de Tours, Inserm, Tours, France.
  • Andres C; Laboratoire de biochimie et biologie moléculaire, CHRU de Tours, France, UMR 1253, iBrain, Université de Tours, Inserm, Tours, France.
  • Blasco H; Laboratoire de biochimie et biologie moléculaire, CHRU de Tours, France, UMR 1253, iBrain, Université de Tours, Inserm, Tours, France.
  • Benz-de Bretagne I; Laboratoire de biochimie et biologie moléculaire, CHRU de Tours, France, UMR 1253, iBrain, Université de Tours, Inserm, Tours, France.
Ann Biol Clin (Paris) ; 77(2): 161-168, 2019 04 01.
Article en Fr | MEDLINE | ID: mdl-30998196
OBJECTIVES: Platelet serotonin and its urinary metabolite 5-HIAA (5-hydroxyindolacetic acid) are the main biomarkers measured for the detection of neuroendocrine tumors (NET). We observe in our laboratory many false positives or false negatives for the 2 assays using threshold values given by the manufacturer. We aim to determine our own local threshold values for a better detection of gastrointestinal NETs. METHODS: We studied patients with measurement of platelet serotonin and/or urinary 5-HIAA in University Hospital of Tours between January 2005 and June 2016. We established an « index ¼ cohort with 75% of patients to determine local threshold value for the 2 parameters. A "validation" cohort constituted with 25% of remaining patients allowed us to compare the performances of manufacturer's values with local threshold values. RESULTS: Two hundred ninety patients were included, with 19 suffering from NETs. Local threshold value for platelet serotonin was determined at 5.13 amol/platelet, the one for urinary 5-HIAA at 3.60 µmol/mmol urinary creatinine. Platelet serotonin specificity was better with local threshold value for identical sensibility (0.75). Urinary 5-HIAA sensibility was improved with local threshold value (1 vs 0.667) for identical specificity (0.902). CONCLUSION: Using our local threshold value for platelet serotonin and urinary 5-HIAA improved diagnostic performances of these biochemical markers to detect NETs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Análisis Químico de la Sangre / Plaquetas / Serotonina / Urinálisis / Tumores Neuroendocrinos / Neoplasias del Sistema Digestivo / Ácido Hidroxiindolacético Tipo de estudio: Diagnostic_studies / Etiology_studies / Incidence_studies / Observational_studies / Risk_factors_studies Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: Fr Revista: Ann Biol Clin (Paris) Año: 2019 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Francia

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Análisis Químico de la Sangre / Plaquetas / Serotonina / Urinálisis / Tumores Neuroendocrinos / Neoplasias del Sistema Digestivo / Ácido Hidroxiindolacético Tipo de estudio: Diagnostic_studies / Etiology_studies / Incidence_studies / Observational_studies / Risk_factors_studies Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: Fr Revista: Ann Biol Clin (Paris) Año: 2019 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Francia