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PELI3 mediates pro-tumor actions of down-regulated miR-365a-5p in non-small cell lung cancer.
He, Yuzheng; Shi, Yantao; Liu, Ruilin; Wang, Zhichao; Wang, Baohua; Li, Shujun; Zhang, Helin.
Afiliación
  • He Y; Department of Thoracic Surgery, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
  • Shi Y; Department of Thoracic Surgery, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
  • Liu R; Department of Thoracic Surgery, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
  • Wang Z; Department of Thoracic Surgery, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
  • Wang B; Department of Thoracic Surgery, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
  • Li S; Department of Thoracic Surgery, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
  • Zhang H; Department of Thoracic Surgery, The Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China. zhl_dr@163.com.
Biol Res ; 52(1): 24, 2019 Apr 17.
Article en En | MEDLINE | ID: mdl-30995936
BACKGROUND: To analyze the relative expression of PELI3 and its mechanistic involvement in the non-small cell lung cancer (NSCLC). METHODS: PELI3 expression in NSCLC tissue samples was determined by the immunohistochemistry. The transcripts abundance of PELI3 was measured with real-time PCR. The protein intensity was analyzed by western blot. The overall survival in respect to PELI3 or miR-365a-5p expression was plotted by the Kaplan-Meier's analysis. Cell growth was determined by colony formation assay. Cell viability was measured by MTT assay. The migration and invasion were evaluated by wound healing and transwell assay respectively. The regulatory effect of miR-365a-5p on PELI3 was interrogated with luciferase reporter assay. The direct binding between miR-365a-5p and PELI3 was analyzed by pulldown assay. RESULTS: PELI3 was aberrantly up-regulated in NSCLC both in vivo and in vitro. High level of PELI3 associated with poor prognosis. PELI3-deficiency significantly inhibited cell viability, colony formation, migration and invasion. We further identified that miR-365a-5p negatively regulated PELI3 in this disease. Ectopic expression of miR-365a-5p in both A549 and H1299 phenocopied PELI3-deficiency. Meanwhile, PELI3-silencing significantly abolished the pro-tumoral effect elicited by miR-365a-5p inhibition. CONCLUSION: Our results highlighted the importance of dysregulated miR-365a-5p-PELI3 signaling axis in NSCLC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regulación hacia Abajo / Carcinoma de Pulmón de Células no Pequeñas / MicroARNs / Ubiquitina-Proteína Ligasas / Neoplasias Pulmonares Límite: Animals / Humans Idioma: En Revista: Biol Res Asunto de la revista: BIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regulación hacia Abajo / Carcinoma de Pulmón de Células no Pequeñas / MicroARNs / Ubiquitina-Proteína Ligasas / Neoplasias Pulmonares Límite: Animals / Humans Idioma: En Revista: Biol Res Asunto de la revista: BIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido